Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.16 NGFR rabbit IgG(H+L) / donkey NGFR 712.13
A375-P3
B8
WM-115 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.23 NGFR rabbit IgG(H+L) / donkey NGFR 968.59
WM115-P3
D8
WM-115 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.82 NGFR rabbit IgG(H+L) / donkey NGFR 2227.68
WM115-P3
B8
WM-115 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.82 NGFR rabbit IgG(H+L) / donkey NGFR 866.72
WM115-P3
E7
A-375 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 112.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 197.43
A375-P2
E4
WM-115 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 112.69 NGFR rabbit IgG(H+L) / donkey NGFR 869.14
WM115-P3
E3
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 113.28 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 16.98
A375-P1
B9
WM-115 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 115.18 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 599.16
WM115-P4
G4
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 115.31 NGFR rabbit IgG(H+L) / donkey NGFR 1535.07
WM115-P3
B5
WM-115 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 117.04 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1192.56
WM115-P4
G2
WM-115 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 117.95 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 206.31
WM115-P2
E8
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.93 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 15.10
A375-P1
C10
A-375 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.57 NGFR rabbit IgG(H+L) / donkey NGFR 119.71
A375-P3
G4
WM-115 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.46 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 61.74
WM115-P2
G2
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.07 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 19.44
A375-P1
C8
COLO 858 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.40 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 450.07
Colo858-P4
E9
COLO 858 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.12 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 317.60
Colo858-P4
F11
WM-115 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.65 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 94.35
WM115-P1
G2
WM-115 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.01 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 157.50
WM115-P1
D3
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.61 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 308.59
Colo858-P4
G10
WM-115 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.71 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 572.25
WM115-P4
C4
WM-115 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.20 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 743.84
WM115-P1
F7
WM-115 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.95 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 194.31
WM115-P2
B8
WM-115 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.00 NGFR rabbit IgG(H+L) / donkey NGFR 854.12
WM115-P3
E2
COLO 858 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.60 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 395.12
Colo858-P4
D9