Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 7.34 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 589.92
A375-P4
C8
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 21.57 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 539.33
A375-P4
C7
A-375 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -22.64 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 229.55
A375-P4
C6
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 9.44 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 383.48
A375-P4
C5
A-375 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 432.27 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 303.65
A375-P2
E8
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 179.36 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 772.74
A375-P4
C4
A-375 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 423.30 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1041.27
A375-P4
C3
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1197.20 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2466.67
A375-P4
C2
A-375 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -73.51 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 244.47
A375-P4
C11
A-375 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 928.51 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 81.57
A375-P2
E7
A-375 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 22.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 197.02
A375-P2
E6
A-375 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 41.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 238.44
A375-P2
E5
A-375 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -53.15 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 663.30
A375-P2
B11
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -35.74 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 516.29
A375-P4
C10
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 13.62 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 609.64
A375-P4
B9
A-375 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 112.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 197.43
A375-P2
E4
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 37.17 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 544.84
A375-P4
B8
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 66.54 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 524.03
A375-P4
B7
A-375 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.93 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 142.85
A375-P2
E3
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 6.50 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 215.67
A375-P4
B6
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 48.57 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 436.46
A375-P4
B5
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 192.80 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 746.57
A375-P4
B4
A-375 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.50 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1074.59
A375-P4
B3
A-375 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 252.32 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 85.10
A375-P2
E2
A-375 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 28.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 346.85
A375-P2
E11