Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 75.31 NGFR rabbit IgG(H+L) / donkey NGFR 1036.92
WM115-P3
D9
WM-115 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 30.80 NGFR rabbit IgG(H+L) / donkey NGFR 1167.95
WM115-P3
E10
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -12.46 NGFR rabbit IgG(H+L) / donkey NGFR 896.89
WM115-P3
E11
WM-115 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.00 NGFR rabbit IgG(H+L) / donkey NGFR 854.12
WM115-P3
E2
WM-115 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 112.69 NGFR rabbit IgG(H+L) / donkey NGFR 869.14
WM115-P3
E3
WM-115 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 72.12 NGFR rabbit IgG(H+L) / donkey NGFR 770.43
WM115-P3
E4
WM-115 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 32.44 NGFR rabbit IgG(H+L) / donkey NGFR 724.88
WM115-P3
E5
WM-115 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 11.76 NGFR rabbit IgG(H+L) / donkey NGFR 666.72
WM115-P3
E6
WM-115 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.82 NGFR rabbit IgG(H+L) / donkey NGFR 866.72
WM115-P3
E7
WM-115 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 107.98 NGFR rabbit IgG(H+L) / donkey NGFR 918.12
WM115-P3
E8
WM-115 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 51.30 NGFR rabbit IgG(H+L) / donkey NGFR 1033.29
WM115-P3
E9
WM-115 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1.36 NGFR rabbit IgG(H+L) / donkey NGFR 979.07
WM115-P3
F10
WM-115 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -20.88 NGFR rabbit IgG(H+L) / donkey NGFR 797.93
WM115-P3
F11
WM-115 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.27 NGFR rabbit IgG(H+L) / donkey NGFR 1110.47
WM115-P3
F2
WM-115 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.54 NGFR rabbit IgG(H+L) / donkey NGFR 976.08
WM115-P3
F3
WM-115 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 89.66 NGFR rabbit IgG(H+L) / donkey NGFR 1051.66
WM115-P3
F4
WM-115 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 58.66 NGFR rabbit IgG(H+L) / donkey NGFR 1134.19
WM115-P3
F5
WM-115 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 26.07 NGFR rabbit IgG(H+L) / donkey NGFR 1001.54
WM115-P3
F6
WM-115 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 95.38 NGFR rabbit IgG(H+L) / donkey NGFR 988.48
WM115-P3
F7
WM-115 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.05 NGFR rabbit IgG(H+L) / donkey NGFR 907.83
WM115-P3
F8
WM-115 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 36.75 NGFR rabbit IgG(H+L) / donkey NGFR 890.13
WM115-P3
F9
WM-115 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 21.85 NGFR rabbit IgG(H+L) / donkey NGFR 1033.43
WM115-P3
G10
WM-115 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -27.37 NGFR rabbit IgG(H+L) / donkey NGFR 768.94
WM115-P3
G11
WM-115 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.02 NGFR rabbit IgG(H+L) / donkey NGFR 1073.92
WM115-P3
G2
WM-115 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.91 NGFR rabbit IgG(H+L) / donkey NGFR 998.17
WM115-P3
G3