Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1220.77 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2378.45
A375-P4
B2
A-375 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -102.60 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 307.17
A375-P4
B11
A-375 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 64.02 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 372.62
A375-P2
E10
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -27.79 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 335.52
A375-P4
B10
A-375 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.08 NGFR rabbit IgG(H+L) / donkey NGFR 132.41
A375-P3
G9
A-375 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.43 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 382.37
A375-P2
D9
A-375 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.08 NGFR rabbit IgG(H+L) / donkey NGFR 90.60
A375-P3
G8
A-375 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 745.91 NGFR rabbit IgG(H+L) / donkey NGFR 245.85
A375-P3
G7
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 86.41 NGFR rabbit IgG(H+L) / donkey NGFR 79.59
A375-P3
G6
A-375 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 98.60 NGFR rabbit IgG(H+L) / donkey NGFR 146.35
A375-P3
G5
A-375 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 382.92 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 293.02
A375-P2
D8
A-375 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 859.17 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 65.80
A375-P2
D7
A-375 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.57 NGFR rabbit IgG(H+L) / donkey NGFR 119.71
A375-P3
G4
A-375 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.38 NGFR rabbit IgG(H+L) / donkey NGFR 97.06
A375-P3
G3
A-375 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -31.18 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 232.59
A375-P2
D6
A-375 Vemurafenib (+)-JQ1 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 374.84 NGFR rabbit IgG(H+L) / donkey NGFR 191.92
A375-P3
G2
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -8.03 NGFR rabbit IgG(H+L) / donkey NGFR 84.94
A375-P3
G11
A-375 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 28.97 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 241.24
A375-P2
D5
A-375 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 40.15 NGFR rabbit IgG(H+L) / donkey NGFR 154.03
A375-P3
G10
A-375 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 180.04 NGFR rabbit IgG(H+L) / donkey NGFR 144.88
A375-P3
F9
A-375 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.95 NGFR rabbit IgG(H+L) / donkey NGFR 93.45
A375-P3
F8
A-375 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 718.83 NGFR rabbit IgG(H+L) / donkey NGFR 240.96
A375-P3
F7
A-375 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.28 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 218.47
A375-P2
D4
A-375 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 191.31 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 155.71
A375-P2
D3
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 46.97 NGFR rabbit IgG(H+L) / donkey NGFR 88.97
A375-P3
F6