Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib I-BET 0.100 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 432.27 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 303.65
A375-P2
E8
A-375 Vemurafenib Belinostat 0.316 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 112.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 197.43
A375-P2
E4
A-375 Vemurafenib I-BET151 3.160 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 291.04
A375-P2
F11
A-375 Vemurafenib (+)-JQ1 0.100 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.87 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 173.78
A375-P2
F3
A-375 Vemurafenib (+)-JQ1 0.316 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 196.41
A375-P2
F4
A-375 Vemurafenib Trametinib 1.000 0.200
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 5.54 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 788.73
A375-P2
B10
A-375 Vemurafenib Trametinib 3.160 0.200
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -53.15 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 663.30
A375-P2
B11
A-375 Vemurafenib 0.100
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 712.85 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 402.29
A375-P2
B3
A-375 Vemurafenib 0.316
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 390.56 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 458.98
A375-P2
B4
A-375 Vemurafenib Trametinib 0.000 0.200
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.55 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 902.23
A375-P2
B7
A-375 Vemurafenib Trametinib 0.100 0.200
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 101.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 860.33
A375-P2
B8
A-375 Vemurafenib Trametinib 1.000 0.200
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 50.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 784.89
A375-P2
C10
A-375 Vemurafenib Trametinib 3.160 0.200
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -18.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 759.90
A375-P2
C11
A-375 Vemurafenib 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 450.58
A375-P2
C5
A-375 Vemurafenib 3.160
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 44.69 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 464.61
A375-P2
C6
A-375 Vemurafenib Trametinib 0.100 0.200
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 71.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 860.61
A375-P2
C8
A-375 Vemurafenib Trametinib 0.316 0.200
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 58.85 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 831.79
A375-P2
C9
A-375 Vemurafenib Belinostat 0.000 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 285.58 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 92.17
A375-P2
D2
A-375 Vemurafenib Belinostat 0.100 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 191.31 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 155.71
A375-P2
D3
A-375 Vemurafenib Belinostat 1.000 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 28.97 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 241.24
A375-P2
D5
A-375 Vemurafenib Belinostat 3.160 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -31.18 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 232.59
A375-P2
D6
A-375 Vemurafenib I-BET 3.160 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 28.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 346.85
A375-P2
E11
A-375 Vemurafenib Belinostat 0.000 1.000
uM
48
hr
2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 252.32 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 85.10
A375-P2
E2
A-375 Vemurafenib I-BET151 0.316 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 226.73 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 298.06
A375-P2
F9
A-375 Vemurafenib I-BET151 0.100 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 426.59 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 226.72
A375-P2
F8