Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 104.85 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 290.79
WM115-P4
B6
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 6.50 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 215.67
A375-P4
B6
WM-115 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 89.26 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 191.67
WM115-P2
B6
COLO 858 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.90 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 513.19
Colo858-P2
B6
COLO 858 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.17 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 68.35
Colo858-P4
B6
WM-115 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 88.70 NGFR rabbit IgG(H+L) / donkey NGFR 1619.14
WM115-P3
B6
COLO 858 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.83 NGFR rabbit IgG(H+L) / donkey NGFR 257.33
Colo858-P3
B6
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.41 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 41.28
A375-P1
B6
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 489.26
A375-P2
B6
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 105.75 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 465.18
WM115-P4
B7
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 87.23 NGFR rabbit IgG(H+L) / donkey NGFR 2203.80
WM115-P3
B7
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 253.13 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 31.77
Colo858-P1
B7
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.84 NGFR rabbit IgG(H+L) / donkey NGFR 788.23
A375-P3
B7
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.22 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 22.58
A375-P1
B7
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 253.58 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 707.88
Colo858-P2
B7
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 205.32 NGFR rabbit IgG(H+L) / donkey NGFR 494.57
Colo858-P3
B7
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 66.54 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 524.03
A375-P4
B7
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 99.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 212.64
WM115-P2
B7
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.55 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 902.23
A375-P2
B7
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 94.16 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 3.35
WM115-P1
B7
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 289.78 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 138.97
Colo858-P4
B7
COLO 858 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 197.93 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 147.08
Colo858-P4
B8
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 37.17 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 544.84
A375-P4
B8
WM-115 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.82 NGFR rabbit IgG(H+L) / donkey NGFR 2227.68
WM115-P3
B8
WM-115 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 76.98 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 2.33
WM115-P1
B8