Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 79.95 NGFR rabbit IgG(H+L) / donkey NGFR 158.37
A375-P3
F5
WM-115 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 269.94 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 158.39
WM115-P1
C3
A-375 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 255.92 NGFR rabbit IgG(H+L) / donkey NGFR 159.75
A375-P3
D8
A-375 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -62.77 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 161.15
A375-P4
E11
COLO 858 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 212.43 NGFR rabbit IgG(H+L) / donkey NGFR 163.78
Colo858-P3
C6
WM-115 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -45.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 164.62
WM115-P2
G11
COLO 858 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.69 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 164.74
Colo858-P4
D11
A-375 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 91.25 NGFR rabbit IgG(H+L) / donkey NGFR 167.27
A375-P3
F10
COLO 858 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 382.99 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 167.43
Colo858-P1
E3
WM-115 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 167.44
WM115-P2
G3
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 224.45 NGFR rabbit IgG(H+L) / donkey NGFR 168.67
Colo858-P3
C5
A-375 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 12.71 NGFR rabbit IgG(H+L) / donkey NGFR 168.79
A375-P3
D11
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -32.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 171.27
WM115-P2
E11
WM-115 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 250.32 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 171.29
WM115-P1
B3
WM-115 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -24.55 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 171.49
WM115-P2
C11
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.94 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 171.85
Colo858-P4
C9
COLO 858 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.46 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 172.15
Colo858-P2
E4
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1317.78 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 172.36
A375-P2
B2
WM-115 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -39.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 172.43
WM115-P2
B11
COLO 858 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.96 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 172.87
Colo858-P4
E11
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1315.15 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 172.98
A375-P2
C2
A-375 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.87 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 173.78
A375-P2
F3
WM-115 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 15.97 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 178.14
WM115-P2
C6
A-375 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -51.45 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 179.22
A375-P4
D11
A-375 Vemurafenib I-BET 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.67 NGFR rabbit IgG(H+L) / donkey NGFR 179.53
A375-P3
E8