Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -8.69 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 241.09
A375-P4
F10
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -8.03 NGFR rabbit IgG(H+L) / donkey NGFR 84.94
A375-P3
G11
WM-115 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -5.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 68.74
WM115-P2
E6
A-375 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -3.64 NGFR rabbit IgG(H+L) / donkey NGFR 380.40
A375-P3
C11
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -3.33 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 289.86
A375-P2
G11
WM-115 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -3.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 92.30
WM115-P2
E5
WM-115 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -2.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 142.13
WM115-P2
F6
WM-115 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -1.15 NGFR rabbit IgG(H+L) / donkey NGFR 2136.86
WM115-P3
B10
A-375 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 0.41 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 438.38
A375-P4
E5
WM-115 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 0.47 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 0.07
WM115-P1
B11
WM-115 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1.05 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 27.78
WM115-P1
F6
WM-115 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1.34 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 509.37
WM115-P4
D5
WM-115 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1.36 NGFR rabbit IgG(H+L) / donkey NGFR 979.07
WM115-P3
F10
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 291.04
A375-P2
F11
COLO 858 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2.87 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 25.49
Colo858-P4
B11
WM-115 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2.89 NGFR rabbit IgG(H+L) / donkey NGFR 1658.33
WM115-P3
C11
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3.01 NGFR rabbit IgG(H+L) / donkey NGFR 76.51
A375-P3
F11
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3.52 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 195.37
WM115-P2
D11
WM-115 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 4.14 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 18.17
WM115-P1
E10
A-375 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 4.70 NGFR rabbit IgG(H+L) / donkey NGFR 24.69
A375-P3
D6
WM-115 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 5.14 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 553.65
WM115-P4
E5
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 5.32 NGFR rabbit IgG(H+L) / donkey NGFR 843.16
WM115-P3
D11
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 5.54 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 788.73
A375-P2
B10
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 6.50 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 215.67
A375-P4
B6
WM-115 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 6.69 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 215.25
WM115-P2
F10