Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -35.12 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) -4.97
WM115-P1
C11
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -32.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 171.27
WM115-P2
E11
A-375 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -31.18 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 232.59
A375-P2
D6
WM-115 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -30.47 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 272.96
WM115-P4
C11
WM-115 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -28.05 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 308.29
WM115-P4
G11
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -27.79 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 335.52
A375-P4
B10
WM-115 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -27.37 NGFR rabbit IgG(H+L) / donkey NGFR 768.94
WM115-P3
G11
A-375 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -27.36 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 235.02
A375-P4
E10
WM-115 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -24.55 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 171.49
WM115-P2
C11
A-375 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -22.64 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 229.55
A375-P4
C6
WM-115 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -22.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 68.18
WM115-P2
D6
WM-115 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -20.88 NGFR rabbit IgG(H+L) / donkey NGFR 797.93
WM115-P3
F11
A-375 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -19.84 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 260.20
A375-P4
D10
WM-115 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -19.46 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 397.38
WM115-P4
E6
A-375 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -18.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 759.90
A375-P2
C11
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -16.26 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 18.54
WM115-P1
E11
WM-115 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -14.81 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 322.49
WM115-P4
F11
WM-115 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -13.59 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 28.88
WM115-P1
G10
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -12.69 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 288.70
WM115-P4
D11
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -12.46 NGFR rabbit IgG(H+L) / donkey NGFR 896.89
WM115-P3
E11
WM-115 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -12.10 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 19.15
WM115-P1
F10
WM-115 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -10.72 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 215.92
WM115-P2
G10
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -10.37 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 445.94
A375-P4
C9
WM-115 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -10.29 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 16.53
WM115-P1
D11
A-375 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -10.01 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 360.55
A375-P2
D11