Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 86.41 NGFR rabbit IgG(H+L) / donkey NGFR 79.59
A375-P3
G6
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 87.23 NGFR rabbit IgG(H+L) / donkey NGFR 2203.80
WM115-P3
B7
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 87.44 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 16.69
A375-P1
B10
A-375 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 88.14 NGFR rabbit IgG(H+L) / donkey NGFR 33.82
A375-P3
D4
WM-115 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 88.70 NGFR rabbit IgG(H+L) / donkey NGFR 1619.14
WM115-P3
B6
WM-115 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 89.26 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 191.67
WM115-P2
B6
A-375 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 89.41 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 298.73
A375-P2
G10
WM-115 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 89.66 NGFR rabbit IgG(H+L) / donkey NGFR 1051.66
WM115-P3
F4
WM-115 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 90.47 NGFR rabbit IgG(H+L) / donkey NGFR 1018.11
WM115-P3
G6
A-375 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 91.25 NGFR rabbit IgG(H+L) / donkey NGFR 167.27
A375-P3
F10
WM-115 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 91.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 223.95
WM115-P2
C5
WM-115 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 91.95 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 23.10
WM115-P1
B6
WM-115 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 92.28 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1486.34
WM115-P4
E7
WM-115 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 92.49 NGFR rabbit IgG(H+L) / donkey NGFR 771.16
WM115-P3
D4
A-375 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 92.75 NGFR rabbit IgG(H+L) / donkey NGFR 779.46
A375-P3
C8
A-375 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 92.77 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 960.83
A375-P4
D3
WM-115 Vemurafenib I-BET 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 93.10 NGFR rabbit IgG(H+L) / donkey NGFR 916.16
WM115-P3
D7
WM-115 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 93.62 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 112.86
WM115-P2
D4
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 94.16 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 3.35
WM115-P1
B7
WM-115 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 95.38 NGFR rabbit IgG(H+L) / donkey NGFR 988.48
WM115-P3
F7
WM-115 Vemurafenib I-BET151 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 96.20 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1432.72
WM115-P4
F7
WM-115 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 96.42 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 84.25
WM115-P2
E3
WM-115 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 97.13 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 657.22
WM115-P4
F4
A-375 Vemurafenib (+)-JQ1 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 98.60 NGFR rabbit IgG(H+L) / donkey NGFR 146.35
A375-P3
G5
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 98.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 288.00
A375-P2
G6