Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 111.40
WM115-P2
B2
WM-115 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 235.84 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 227.32
WM115-P2
B3
WM-115 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.26 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 227.60
WM115-P2
B4
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 107.90 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 207.30
WM115-P2
B5
WM-115 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 89.26 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 191.67
WM115-P2
B6
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.75 NGFR rabbit IgG(H+L) / donkey NGFR 648.96
A375-P3
C5
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 311.66 NGFR rabbit IgG(H+L) / donkey NGFR 695.43
A375-P3
C4
A-375 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 488.72 NGFR rabbit IgG(H+L) / donkey NGFR 484.00
A375-P3
C3
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1265.78 NGFR rabbit IgG(H+L) / donkey NGFR 358.34
A375-P3
C2
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.56 NGFR rabbit IgG(H+L) / donkey NGFR 435.84
A375-P3
B6
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 324.06 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 110.35
WM115-P2
C2
WM-115 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 237.92 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 209.32
WM115-P2
C3
WM-115 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 242.39
WM115-P2
C4
WM-115 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 91.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 223.95
WM115-P2
C5
WM-115 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 15.97 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 178.14
WM115-P2
C6
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.17 NGFR rabbit IgG(H+L) / donkey NGFR 653.89
A375-P3
B5
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 325.95 NGFR rabbit IgG(H+L) / donkey NGFR 687.57
A375-P3
B4
A-375 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 586.19 NGFR rabbit IgG(H+L) / donkey NGFR 493.73
A375-P3
B3
A-375 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 44.69 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 464.61
A375-P2
C6
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1226.36 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 797.35
Colo858-P1
B2