Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 220.94 NGFR rabbit IgG(H+L) / donkey NGFR 94.00
Colo858-P3
D5
A-375 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 219.54 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 47.32
A375-P1
D9
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 218.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 228.57
Colo858-P2
G10
COLO 858 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 218.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 295.01
Colo858-P2
E9
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 217.21 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 146.42
Colo858-P4
B9
A-375 Vemurafenib I-BET151 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 216.96 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 286.88
A375-P2
G9
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 216.87 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 726.06
Colo858-P2
C10
A-375 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 215.80 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 146.91
A375-P1
D3
COLO 858 Vemurafenib Belinostat 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 215.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 117.26
Colo858-P2
E5
COLO 858 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 213.69 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 39.87
Colo858-P1
E6
A-375 Vemurafenib I-BET 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 212.70 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 41.38
A375-P1
E9
COLO 858 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 212.43 NGFR rabbit IgG(H+L) / donkey NGFR 163.78
Colo858-P3
C6
COLO 858 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 211.72 NGFR rabbit IgG(H+L) / donkey NGFR 68.81
Colo858-P3
E6
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 210.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 287.24
Colo858-P2
D10
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 210.18 NGFR rabbit IgG(H+L) / donkey NGFR 376.06
Colo858-P3
C9
COLO 858 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 209.32 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 478.41
Colo858-P2
C6
COLO 858 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 208.84 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 27.54
Colo858-P1
B11
COLO 858 Vemurafenib I-BET151 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 207.25 NGFR rabbit IgG(H+L) / donkey NGFR 32.37
Colo858-P3
F8
WM-115 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 206.55 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1090.63
WM115-P4
B3
A-375 Vemurafenib (+)-JQ1 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 206.48 NGFR rabbit IgG(H+L) / donkey NGFR 104.55
A375-P3
F3
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 205.32 NGFR rabbit IgG(H+L) / donkey NGFR 494.57
Colo858-P3
B7
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 205.19 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 738.57
Colo858-P2
C7
COLO 858 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 205.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 290.14
Colo858-P2
E11
COLO 858 Vemurafenib (+)-JQ1 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 204.72 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 916.68
Colo858-P4
F4
COLO 858 Vemurafenib I-BET151 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 204.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 192.98
Colo858-P2
F10