Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 49.69 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 29.92
A375-P1
G11
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3.01 NGFR rabbit IgG(H+L) / donkey NGFR 76.51
A375-P3
F11
A-375 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 15.40 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 34.33
A375-P1
D6
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -3.33 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 289.86
A375-P2
G11
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.41 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 41.28
A375-P1
B6
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.47 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 56.09
A375-P1
G6
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 98.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 288.00
A375-P2
G6
COLO 858 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.92 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 285.28
Colo858-P2
D11
A-375 Vemurafenib (+)-JQ1 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 46.97 NGFR rabbit IgG(H+L) / donkey NGFR 88.97
A375-P3
F6
COLO 858 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.26 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 90.70
Colo858-P2
E6
A-375 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -53.15 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 663.30
A375-P2
B11
A-375 Vemurafenib I-BET151 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -8.03 NGFR rabbit IgG(H+L) / donkey NGFR 84.94
A375-P3
G11
A-375 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 63.28 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 30.98
A375-P1
E11
A-375 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 44.69 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 464.61
A375-P2
C6
A-375 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 39.13 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 9.87
A375-P1
B11
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 489.26
A375-P2
B6
A-375 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.57 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 45.79
A375-P1
C6
A-375 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.56 NGFR rabbit IgG(H+L) / donkey NGFR 435.84
A375-P3
B6
A-375 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -73.51 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 244.47
A375-P4
C11
A-375 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -22.64 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 229.55
A375-P4
C6
A-375 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -18.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 759.90
A375-P2
C11
A-375 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 22.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 197.02
A375-P2
E6
A-375 Vemurafenib Belinostat 3.160 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 28.55 ERK-1 (pT202; pY204); ERK-1 (pT202); ERK-2 (pT185; pY187); ERK-2 (pT185) rabbit IgG(H+L) / donkey ERK-1 (pT202; pY204) ERK-1 (pT202) ERK-2 (pT185; pY187) ERK-2 (pT185) 42.08
A375-P1
E6
COLO 858 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 77.68 NGFR rabbit IgG(H+L) / donkey NGFR 102.40
Colo858-P3
C11
COLO 858 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.83 NGFR rabbit IgG(H+L) / donkey NGFR 257.33
Colo858-P3
B6