Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 4 of 4) - Dataset (ID:20277)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein 1 Detected (647) Protein 2 Detected (647) Protein 3 Detected (647) Protein 4 Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.06 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 622.29
Colo858-P2
B10
COLO 858 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 352.27
Colo858-P2
B11
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1420.93 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 94.42
Colo858-P2
B2
COLO 858 Vemurafenib 0.100
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 792.50 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 412.94
Colo858-P2
B3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 414.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 471.30
Colo858-P2
B4
COLO 858 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 323.76 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 570.77
Colo858-P2
B5
COLO 858 Vemurafenib 3.160
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.90 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 513.19
Colo858-P2
B6
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 253.58 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 707.88
Colo858-P2
B7
COLO 858 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 312.37 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 722.56
Colo858-P2
B8
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 643.00
Colo858-P2
B9
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 216.87 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 726.06
Colo858-P2
C10
COLO 858 Vemurafenib Trametinib 3.160 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 433.21
Colo858-P2
C11
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1478.74 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 103.01
Colo858-P2
C2
COLO 858 Vemurafenib 0.100
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 813.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 449.08
Colo858-P2
C3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 412.31 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 482.64
Colo858-P2
C4
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 268.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 540.81
Colo858-P2
C5
COLO 858 Vemurafenib 3.160
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 209.32 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 478.41
Colo858-P2
C6
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 205.19 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 738.57
Colo858-P2
C7
COLO 858 Vemurafenib Trametinib 0.100 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 236.82 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 711.12
Colo858-P2
C8
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 242.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 749.04
Colo858-P2
C9
COLO 858 Vemurafenib I-BET 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 210.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 287.24
Colo858-P2
D10
COLO 858 Vemurafenib I-BET 3.160 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.92 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 285.28
Colo858-P2
D11
COLO 858 Vemurafenib Belinostat 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 405.61 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 193.33
Colo858-P2
D2
COLO 858 Vemurafenib Belinostat 0.100 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 377.67 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 234.61
Colo858-P2
D3
COLO 858 Vemurafenib Belinostat 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 137.68
Colo858-P2
D4