Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2268 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 202.77 NGFR rabbit IgG(H+L) / donkey NGFR 187.51
A375-B2-rep1
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1248 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.32 NGFR rabbit IgG(H+L) / donkey NGFR 137.61
A375-B1-rep2
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1159 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.23 NGFR rabbit IgG(H+L) / donkey NGFR 133.62
A375-B1-rep2
H8
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1100 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.49 NGFR rabbit IgG(H+L) / donkey NGFR 149.00
A375-B1-rep2
H7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1114 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.61 NGFR rabbit IgG(H+L) / donkey NGFR 154.43
A375-B1-rep2
H6
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1126 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 214.28 NGFR rabbit IgG(H+L) / donkey NGFR 175.81
A375-B1-rep2
H5
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1303 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 204.72 NGFR rabbit IgG(H+L) / donkey NGFR 152.30
A375-B1-rep2
H4
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1421 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 197.45 NGFR rabbit IgG(H+L) / donkey NGFR 159.25
A375-B1-rep2
H3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1542 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.78 NGFR rabbit IgG(H+L) / donkey NGFR 167.11
A375-B1-rep2
H2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1364 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 201.35 NGFR rabbit IgG(H+L) / donkey NGFR 138.83
A375-B1-rep2
H11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1429 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.39 NGFR rabbit IgG(H+L) / donkey NGFR 144.00
A375-B1-rep2
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.71 NGFR rabbit IgG(H+L) / donkey NGFR 826.75
WM115-A2-rep2
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 867 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.77 NGFR rabbit IgG(H+L) / donkey NGFR 905.23
WM115-A2-rep2
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 991 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.39 NGFR rabbit IgG(H+L) / donkey NGFR 912.83
WM115-A2-rep2
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 854 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.28 NGFR rabbit IgG(H+L) / donkey NGFR 899.54
WM115-A2-rep2
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 808 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.65 NGFR rabbit IgG(H+L) / donkey NGFR 855.37
WM115-A2-rep2
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 754 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.51 NGFR rabbit IgG(H+L) / donkey NGFR 910.39
WM115-A2-rep2
H5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.80 NGFR rabbit IgG(H+L) / donkey NGFR 893.83
WM115-A2-rep2
H6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 810 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.85 NGFR rabbit IgG(H+L) / donkey NGFR 859.67
WM115-A2-rep2
H7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 845 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.28 NGFR rabbit IgG(H+L) / donkey NGFR 900.40
WM115-A2-rep2
H8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 836 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.96 NGFR rabbit IgG(H+L) / donkey NGFR 867.95
WM115-A2-rep2
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1238 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.53 NGFR rabbit IgG(H+L) / donkey NGFR 1143.34
WM115-B1-rep1
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1082 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.35 NGFR rabbit IgG(H+L) / donkey NGFR 1063.55
WM115-B1-rep1
A11