Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 898 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.58 NGFR rabbit IgG(H+L) / donkey NGFR 797.92
WM115-A2-rep1
H5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1094 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.83 NGFR rabbit IgG(H+L) / donkey NGFR 922.23
WM115-A2-rep1
H6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 909 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 131.83 NGFR rabbit IgG(H+L) / donkey NGFR 973.88
WM115-A2-rep1
H7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1040 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.93 NGFR rabbit IgG(H+L) / donkey NGFR 791.91
WM115-A2-rep1
H8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1026 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 115.53 NGFR rabbit IgG(H+L) / donkey NGFR 936.58
WM115-A2-rep1
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1372 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.88 NGFR rabbit IgG(H+L) / donkey NGFR 973.52
WM115-A2-rep2
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1297 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.03 NGFR rabbit IgG(H+L) / donkey NGFR 965.78
WM115-A2-rep2
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1422 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.16 NGFR rabbit IgG(H+L) / donkey NGFR 882.89
WM115-A2-rep2
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1319 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.08 NGFR rabbit IgG(H+L) / donkey NGFR 923.84
WM115-A2-rep2
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1454 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.37 NGFR rabbit IgG(H+L) / donkey NGFR 947.23
WM115-A2-rep2
A4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1502 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.53 NGFR rabbit IgG(H+L) / donkey NGFR 933.73
WM115-A2-rep2
A5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1304 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.88 NGFR rabbit IgG(H+L) / donkey NGFR 937.20
WM115-A2-rep2
A6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1398 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.64 NGFR rabbit IgG(H+L) / donkey NGFR 1025.20
WM115-A2-rep2
A7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1445 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.41 NGFR rabbit IgG(H+L) / donkey NGFR 1020.33
WM115-A2-rep2
A8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1643 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 131.59 NGFR rabbit IgG(H+L) / donkey NGFR 1021.18
WM115-A2-rep2
A9
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2657 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.03 NGFR rabbit IgG(H+L) / donkey NGFR 182.34
A375-B2-rep1
A9
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2285 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.90 NGFR rabbit IgG(H+L) / donkey NGFR 189.57
A375-B2-rep1
A8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1141 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.91 NGFR rabbit IgG(H+L) / donkey NGFR 1132.64
WM115-B2-rep2
H5
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2671 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 196.90 NGFR rabbit IgG(H+L) / donkey NGFR 192.72
A375-B2-rep1
A7
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2542 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.76 NGFR rabbit IgG(H+L) / donkey NGFR 190.67
A375-B2-rep1
A6
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2345 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.31 NGFR rabbit IgG(H+L) / donkey NGFR 193.43
A375-B2-rep1
A5
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2754 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 179.51 NGFR rabbit IgG(H+L) / donkey NGFR 205.98
A375-B2-rep1
A4
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2443 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.77 NGFR rabbit IgG(H+L) / donkey NGFR 168.94
A375-B2-rep1
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2478 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.11 NGFR rabbit IgG(H+L) / donkey NGFR 175.65
A375-B2-rep1
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2280 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 223.55 NGFR rabbit IgG(H+L) / donkey NGFR 187.85
A375-B2-rep1
A11