Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Azacitidine 0.3160 0.1067
uM
 48
hr
 2 2234 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.09 NGFR rabbit IgG(H+L) / donkey NGFR 137.68
Colo858-B2-rep2
E2
COLO 858 Vemurafenib Azacitidine 0.3160 0.1067
uM
 48
hr
 1 1758 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.56 NGFR rabbit IgG(H+L) / donkey NGFR 128.93
Colo858-B2-rep1
E2
A-375 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 1 1210 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 261.75 NGFR rabbit IgG(H+L) / donkey NGFR 53.65
A375-A2-rep1
E2
COLO 858 Vemurafenib Azacitidine 0.3160 2.6667
uM
 48
hr
 1 840 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.97 NGFR rabbit IgG(H+L) / donkey NGFR 20.08
Colo858-A2-rep1
E2
A-375 Vemurafenib Belinostat 1.0000 0.5333
uM
 48
hr
 2 2075 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.98 NGFR rabbit IgG(H+L) / donkey NGFR 20.46
A375-B1-rep2
C4
A-375 Vemurafenib Belinostat 1.0000 0.1067
uM
 48
hr
 2 2898 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.66 NGFR rabbit IgG(H+L) / donkey NGFR 44.08
A375-B2-rep2
C4
COLO 858 Vemurafenib Belinostat 0.3160 2.6667
uM
 48
hr
 2 165 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 207.48 NGFR rabbit IgG(H+L) / donkey NGFR 20.78
Colo858-A2-rep2
C4
A-375 Vemurafenib Belinostat 1.0000 0.5333
uM
 48
hr
 1 1911 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.06 NGFR rabbit IgG(H+L) / donkey NGFR 19.85
A375-B1-rep1
C4
WM-115 Vemurafenib Belinostat 1.0000 0.5333
uM
 48
hr
 1 1138 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.07 NGFR rabbit IgG(H+L) / donkey NGFR 352.75
WM115-B1-rep1
C4
WM-115 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 1 705 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.59 NGFR rabbit IgG(H+L) / donkey NGFR 342.51
WM115-A2-rep1
C4
COLO 858 Vemurafenib Belinostat 0.3160 0.1067
uM
 48
hr
 1 798 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 187.52 NGFR rabbit IgG(H+L) / donkey NGFR 66.34
Colo858-B2-rep1
C4
A-375 Vemurafenib Belinostat 1.0000 0.1067
uM
 48
hr
 1 2949 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.46 NGFR rabbit IgG(H+L) / donkey NGFR 37.06
A375-B2-rep1
C4
COLO 858 Vemurafenib Belinostat 0.3160 0.5333
uM
 48
hr
 2 367 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 240.33 NGFR rabbit IgG(H+L) / donkey NGFR 12.62
Colo858-B1-rep2
C4
COLO 858 Vemurafenib Belinostat 0.3160 0.1067
uM
 48
hr
 2 796 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.45 NGFR rabbit IgG(H+L) / donkey NGFR 129.48
Colo858-B2-rep2
C4
A-375 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 1 279 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 395.49 NGFR rabbit IgG(H+L) / donkey NGFR 21.95
A375-A2-rep1
C4
WM-115 Vemurafenib Belinostat 1.0000 0.1067
uM
 48
hr
 2 1389 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.46 NGFR rabbit IgG(H+L) / donkey NGFR 499.87
WM115-B2-rep2
C4
WM-115 Vemurafenib Belinostat 1.0000 0.1067
uM
 48
hr
 1 1567 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.29 NGFR rabbit IgG(H+L) / donkey NGFR 524.82
WM115-B2-rep1
C4
WM-115 Vemurafenib Belinostat 1.0000 0.5333
uM
 48
hr
 2 1206 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.06 NGFR rabbit IgG(H+L) / donkey NGFR 358.18
WM115-B1-rep2
C4
A-375 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 2 319 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 360.58 NGFR rabbit IgG(H+L) / donkey NGFR 26.94
A375-A2-rep2
C4
COLO 858 Vemurafenib Belinostat 0.3160 0.5333
uM
 48
hr
 1 394 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.28 NGFR rabbit IgG(H+L) / donkey NGFR 28.69
Colo858-B1-rep1
C4
COLO 858 Vemurafenib Belinostat 0.3160 2.6667
uM
 48
hr
 1 209 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 309.25 NGFR rabbit IgG(H+L) / donkey NGFR 21.49
Colo858-A2-rep1
C4
WM-115 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 2 690 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.25 NGFR rabbit IgG(H+L) / donkey NGFR 284.20
WM115-A2-rep2
C4
A-375 Vemurafenib BIX-01294 1.0000 0.1067
uM
 48
hr
 1 2703 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.26 NGFR rabbit IgG(H+L) / donkey NGFR 174.07
A375-B2-rep1
F5
A-375 Vemurafenib BIX-01294 1.0000 2.6667
uM
 48
hr
 2 1250 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 292.19 NGFR rabbit IgG(H+L) / donkey NGFR 42.51
A375-A2-rep2
F5
COLO 858 Vemurafenib BIX-01294 0.3160 0.1067
uM
 48
hr
 1 2175 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 374.62 NGFR rabbit IgG(H+L) / donkey NGFR 150.11
Colo858-B2-rep1
F5