Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 1 1493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.17 NGFR rabbit IgG(H+L) / donkey NGFR 1162.33
WM115-B2-rep1
B6
A-375 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 1 2690 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 178.62 NGFR rabbit IgG(H+L) / donkey NGFR 187.40
A375-B2-rep1
B6
COLO 858 Vemurafenib GSK-J2 0.3160 2.6667
uM
 48
hr
 1 2652 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.90 NGFR rabbit IgG(H+L) / donkey NGFR 155.80
Colo858-A2-rep1
C6
WM-115 Vemurafenib GSK-J2 1.0000 0.5333
uM
 48
hr
 1 1420 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.29 NGFR rabbit IgG(H+L) / donkey NGFR 997.01
WM115-B1-rep1
C6
COLO 858 Vemurafenib GSK-J2 0.3160 0.1067
uM
 48
hr
 2 2915 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.92 NGFR rabbit IgG(H+L) / donkey NGFR 159.25
Colo858-B2-rep2
C6
A-375 Vemurafenib GSK-J2 1.0000 0.5333
uM
 48
hr
 2 3124 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.22 NGFR rabbit IgG(H+L) / donkey NGFR 175.54
A375-B1-rep2
C6
A-375 Vemurafenib GSK-J2 1.0000 0.1067
uM
 48
hr
 2 2747 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.01 NGFR rabbit IgG(H+L) / donkey NGFR 157.50
A375-B2-rep2
C6
COLO 858 Vemurafenib GSK-J2 0.3160 2.6667
uM
 48
hr
 2 2739 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 331.72 NGFR rabbit IgG(H+L) / donkey NGFR 152.80
Colo858-A2-rep2
C6
A-375 Vemurafenib GSK-J2 1.0000 2.6667
uM
 48
hr
 1 2997 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.28 NGFR rabbit IgG(H+L) / donkey NGFR 199.06
A375-A2-rep1
C6
WM-115 Vemurafenib GSK-J2 1.0000 2.6667
uM
 48
hr
 2 1641 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 117.71 NGFR rabbit IgG(H+L) / donkey NGFR 1004.18
WM115-A2-rep2
C6
WM-115 Vemurafenib GSK-J2 1.0000 0.5333
uM
 48
hr
 2 1550 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.34 NGFR rabbit IgG(H+L) / donkey NGFR 1141.48
WM115-B1-rep2
C6
WM-115 Vemurafenib GSK-J2 1.0000 2.6667
uM
 48
hr
 1 1446 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.27 NGFR rabbit IgG(H+L) / donkey NGFR 1076.83
WM115-A2-rep1
C6
A-375 Vemurafenib GSK-J2 1.0000 2.6667
uM
 48
hr
 2 3289 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.33 NGFR rabbit IgG(H+L) / donkey NGFR 210.26
A375-A2-rep2
C6
COLO 858 Vemurafenib GSK-J2 0.3160 0.5333
uM
 48
hr
 2 2677 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 312.56 NGFR rabbit IgG(H+L) / donkey NGFR 142.25
Colo858-B1-rep2
C6
A-375 Vemurafenib GSK-J2 1.0000 0.5333
uM
 48
hr
 1 3274 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.25 NGFR rabbit IgG(H+L) / donkey NGFR 219.64
A375-B1-rep1
C6
COLO 858 Vemurafenib GSK-J2 0.3160 0.1067
uM
 48
hr
 1 2462 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 317.12 NGFR rabbit IgG(H+L) / donkey NGFR 150.12
Colo858-B2-rep1
C6
WM-115 Vemurafenib GSK-J2 1.0000 0.1067
uM
 48
hr
 2 1417 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.53 NGFR rabbit IgG(H+L) / donkey NGFR 1067.45
WM115-B2-rep2
C6
COLO 858 Vemurafenib GSK-J2 0.3160 0.5333
uM
 48
hr
 1 2483 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 312.20 NGFR rabbit IgG(H+L) / donkey NGFR 136.57
Colo858-B1-rep1
C6
WM-115 Vemurafenib GSK-J2 1.0000 0.1067
uM
 48
hr
 1 1729 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.85 NGFR rabbit IgG(H+L) / donkey NGFR 1178.59
WM115-B2-rep1
C6
A-375 Vemurafenib GSK-J2 1.0000 0.1067
uM
 48
hr
 1 3266 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 196.57 NGFR rabbit IgG(H+L) / donkey NGFR 202.00
A375-B2-rep1
C6
COLO 858 Vemurafenib GSK-J4 0.3160 2.6667
uM
 48
hr
 1 528 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 232.59 NGFR rabbit IgG(H+L) / donkey NGFR 14.66
Colo858-A2-rep1
D6
A-375 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 1 1533 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.60 NGFR rabbit IgG(H+L) / donkey NGFR 96.94
A375-A2-rep1
D6
WM-115 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 2 1545 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.21 NGFR rabbit IgG(H+L) / donkey NGFR 1027.05
WM115-A2-rep2
D6
COLO 858 Vemurafenib GSK-J4 0.3160 0.1067
uM
 48
hr
 1 3271 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 334.17 NGFR rabbit IgG(H+L) / donkey NGFR 153.69
Colo858-B2-rep1
D6
WM-115 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 1 1461 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.76 NGFR rabbit IgG(H+L) / donkey NGFR 1082.05
WM115-A2-rep1
D6