Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Resveratrol 0.3160 2.6667
uM
 48
hr
 1 2795 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.85 NGFR rabbit IgG(H+L) / donkey NGFR 129.75
Colo858-A2-rep1
E5
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 955 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.67 NGFR rabbit IgG(H+L) / donkey NGFR 131.81
A375-A2-rep1
H5
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 2.6667
uM
 48
hr
 1 3105 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.79 NGFR rabbit IgG(H+L) / donkey NGFR 132.41
Colo858-A2-rep1
E8
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1834 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.77 NGFR rabbit IgG(H+L) / donkey NGFR 166.59
A375-A2-rep1
A10
COLO 858 Vemurafenib Garcinol 0.3160 2.6667
uM
 48
hr
 1 981 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 330.96 NGFR rabbit IgG(H+L) / donkey NGFR 147.82
Colo858-A2-rep1
F3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 969 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.13 NGFR rabbit IgG(H+L) / donkey NGFR 146.47
A375-A2-rep1
H6
COLO 858 Vemurafenib I-BET151 0.3160 2.6667
uM
 48
hr
 1 1486 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.87 NGFR rabbit IgG(H+L) / donkey NGFR 7.82
Colo858-A2-rep1
F7
A-375 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 1 1533 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.60 NGFR rabbit IgG(H+L) / donkey NGFR 96.94
A375-A2-rep1
D6
COLO 858 Vemurafenib RG-108 0.3160 2.6667
uM
 48
hr
 1 1471 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 320.76 NGFR rabbit IgG(H+L) / donkey NGFR 126.71
Colo858-A2-rep1
G2
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1074 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.31 NGFR rabbit IgG(H+L) / donkey NGFR 161.00
A375-A2-rep1
H7
COLO 858 Vemurafenib UNC0638 0.3160 2.6667
uM
 48
hr
 1 581 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 215.25 NGFR rabbit IgG(H+L) / donkey NGFR 98.55
Colo858-A2-rep1
G5
COLO 858 Vemurafenib Ischemin 0.3160 2.6667
uM
 48
hr
 1 1323 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.48 NGFR rabbit IgG(H+L) / donkey NGFR 114.56
Colo858-A2-rep1
G7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 854 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 320.42 NGFR rabbit IgG(H+L) / donkey NGFR 127.24
Colo858-A2-rep1
H10
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1099 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.81 NGFR rabbit IgG(H+L) / donkey NGFR 136.50
A375-A2-rep1
H8
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2214 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.79 NGFR rabbit IgG(H+L) / donkey NGFR 205.29
A375-A2-rep1
A2
A-375 Vemurafenib (-)-JQ1 1.0000 2.6667
uM
 48
hr
 1 3035 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.74 NGFR rabbit IgG(H+L) / donkey NGFR 239.66
A375-A2-rep1
D7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 797 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.09 NGFR rabbit IgG(H+L) / donkey NGFR 127.30
Colo858-A2-rep1
H6
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1087 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.27 NGFR rabbit IgG(H+L) / donkey NGFR 138.42
A375-A2-rep1
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2268 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 202.77 NGFR rabbit IgG(H+L) / donkey NGFR 187.51
A375-B2-rep1
A10
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2280 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 223.55 NGFR rabbit IgG(H+L) / donkey NGFR 187.85
A375-B2-rep1
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2478 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.11 NGFR rabbit IgG(H+L) / donkey NGFR 175.65
A375-B2-rep1
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2443 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.77 NGFR rabbit IgG(H+L) / donkey NGFR 168.94
A375-B2-rep1
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2754 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 179.51 NGFR rabbit IgG(H+L) / donkey NGFR 205.98
A375-B2-rep1
A4
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2345 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.31 NGFR rabbit IgG(H+L) / donkey NGFR 193.43
A375-B2-rep1
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1008 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 371.10 NGFR rabbit IgG(H+L) / donkey NGFR 120.59
Colo858-B1-rep1
A5