Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1040 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.93 NGFR rabbit IgG(H+L) / donkey NGFR 791.91
WM115-A2-rep1
H8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 876 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.53 NGFR rabbit IgG(H+L) / donkey NGFR 1049.12
WM115-B2-rep1
H8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 793 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 260.38 NGFR rabbit IgG(H+L) / donkey NGFR 122.78
Colo858-A2-rep1
H8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 895 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.80 NGFR rabbit IgG(H+L) / donkey NGFR 971.63
WM115-B1-rep2
H8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 983 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 275.00 NGFR rabbit IgG(H+L) / donkey NGFR 146.13
Colo858-B2-rep1
H8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1265 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.56 NGFR rabbit IgG(H+L) / donkey NGFR 114.69
Colo858-B1-rep1
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 977 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.61 NGFR rabbit IgG(H+L) / donkey NGFR 118.55
Colo858-A2-rep1
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 780 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 234.33 NGFR rabbit IgG(H+L) / donkey NGFR 134.94
Colo858-A2-rep2
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 945 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 235.64 NGFR rabbit IgG(H+L) / donkey NGFR 135.45
Colo858-B1-rep2
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 836 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.96 NGFR rabbit IgG(H+L) / donkey NGFR 867.95
WM115-A2-rep2
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 271.95 NGFR rabbit IgG(H+L) / donkey NGFR 134.54
Colo858-B2-rep2
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1550 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 209.64 NGFR rabbit IgG(H+L) / donkey NGFR 158.29
A375-A2-rep2
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 906 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.77 NGFR rabbit IgG(H+L) / donkey NGFR 1117.05
WM115-B2-rep1
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1248 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.32 NGFR rabbit IgG(H+L) / donkey NGFR 137.61
A375-B1-rep2
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1514 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.99 NGFR rabbit IgG(H+L) / donkey NGFR 137.41
A375-B2-rep2
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1118 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.18 NGFR rabbit IgG(H+L) / donkey NGFR 1039.92
WM115-B2-rep2
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 949 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 297.75 NGFR rabbit IgG(H+L) / donkey NGFR 140.37
Colo858-B2-rep1
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1026 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 115.53 NGFR rabbit IgG(H+L) / donkey NGFR 936.58
WM115-A2-rep1
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1733 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.19 NGFR rabbit IgG(H+L) / donkey NGFR 146.36
A375-B2-rep1
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1087 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.27 NGFR rabbit IgG(H+L) / donkey NGFR 138.42
A375-A2-rep1
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1086 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.77 NGFR rabbit IgG(H+L) / donkey NGFR 1029.13
WM115-B1-rep1
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 827 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.26 NGFR rabbit IgG(H+L) / donkey NGFR 870.16
WM115-B1-rep2
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1314 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.73 NGFR rabbit IgG(H+L) / donkey NGFR 154.69
A375-B1-rep1
H9