Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib RG-108 1.0000 2.6667
uM
 48
hr
 1 1476 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 171.86 NGFR rabbit IgG(H+L) / donkey NGFR 162.39
A375-A2-rep1
G2
WM-115 Vemurafenib RG-108 1.0000 0.5333
uM
 48
hr
 2 1105 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.17 NGFR rabbit IgG(H+L) / donkey NGFR 1056.85
WM115-B1-rep2
G2
A-375 Vemurafenib RG-108 1.0000 0.5333
uM
 48
hr
 1 1759 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.66 NGFR rabbit IgG(H+L) / donkey NGFR 137.19
A375-B1-rep1
G2
COLO 858 Vemurafenib Anacardic acid 0.3160 0.1067
uM
 48
hr
 1 1144 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 317.68 NGFR rabbit IgG(H+L) / donkey NGFR 153.35
Colo858-B2-rep1
G3
COLO 858 Vemurafenib Anacardic acid 0.3160 0.5333
uM
 48
hr
 1 1751 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 322.89 NGFR rabbit IgG(H+L) / donkey NGFR 153.60
Colo858-B1-rep1
G3
WM-115 Vemurafenib Anacardic acid 1.0000 2.6667
uM
 48
hr
 1 997 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.62 NGFR rabbit IgG(H+L) / donkey NGFR 928.34
WM115-A2-rep1
G3
A-375 Vemurafenib Anacardic acid 1.0000 0.1067
uM
 48
hr
 2 2049 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 170.49 NGFR rabbit IgG(H+L) / donkey NGFR 140.28
A375-B2-rep2
G3
COLO 858 Vemurafenib Anacardic acid 0.3160 0.1067
uM
 48
hr
 2 1570 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.62 NGFR rabbit IgG(H+L) / donkey NGFR 122.24
Colo858-B2-rep2
G3
WM-115 Vemurafenib Anacardic acid 1.0000 2.6667
uM
 48
hr
 2 1013 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.50 NGFR rabbit IgG(H+L) / donkey NGFR 989.81
WM115-A2-rep2
G3
WM-115 Vemurafenib Anacardic acid 1.0000 0.1067
uM
 48
hr
 1 1092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.00 NGFR rabbit IgG(H+L) / donkey NGFR 967.15
WM115-B2-rep1
G3
A-375 Vemurafenib Anacardic acid 1.0000 0.5333
uM
 48
hr
 2 1745 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.73 NGFR rabbit IgG(H+L) / donkey NGFR 158.90
A375-B1-rep2
G3
COLO 858 Vemurafenib Anacardic acid 0.3160 2.6667
uM
 48
hr
 1 1253 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.28 NGFR rabbit IgG(H+L) / donkey NGFR 120.19
Colo858-A2-rep1
G3
COLO 858 Vemurafenib Anacardic acid 0.3160 0.5333
uM
 48
hr
 2 926 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.48 NGFR rabbit IgG(H+L) / donkey NGFR 124.30
Colo858-B1-rep2
G3
A-375 Vemurafenib Anacardic acid 1.0000 2.6667
uM
 48
hr
 2 1845 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 197.52 NGFR rabbit IgG(H+L) / donkey NGFR 193.43
A375-A2-rep2
G3
A-375 Vemurafenib Anacardic acid 1.0000 0.5333
uM
 48
hr
 1 1888 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.64 NGFR rabbit IgG(H+L) / donkey NGFR 136.19
A375-B1-rep1
G3
COLO 858 Vemurafenib Anacardic acid 0.3160 2.6667
uM
 48
hr
 2 998 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 267.99 NGFR rabbit IgG(H+L) / donkey NGFR 163.66
Colo858-A2-rep2
G3
WM-115 Vemurafenib Anacardic acid 1.0000 0.5333
uM
 48
hr
 1 1099 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.32 NGFR rabbit IgG(H+L) / donkey NGFR 1027.42
WM115-B1-rep1
G3
WM-115 Vemurafenib Anacardic acid 1.0000 0.5333
uM
 48
hr
 2 860 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.06 NGFR rabbit IgG(H+L) / donkey NGFR 1034.36
WM115-B1-rep2
G3
A-375 Vemurafenib Anacardic acid 1.0000 2.6667
uM
 48
hr
 1 1318 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.31 NGFR rabbit IgG(H+L) / donkey NGFR 170.13
A375-A2-rep1
G3
A-375 Vemurafenib Anacardic acid 1.0000 0.1067
uM
 48
hr
 1 1913 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.69 NGFR rabbit IgG(H+L) / donkey NGFR 176.01
A375-B2-rep1
G3
WM-115 Vemurafenib Anacardic acid 1.0000 0.1067
uM
 48
hr
 2 1318 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.59 NGFR rabbit IgG(H+L) / donkey NGFR 1134.58
WM115-B2-rep2
G3
A-375 Vemurafenib MC1568 1.0000 2.6667
uM
 48
hr
 1 1904 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 389.68 NGFR rabbit IgG(H+L) / donkey NGFR 146.07
A375-A2-rep1
G4
COLO 858 Vemurafenib MC1568 0.3160 0.5333
uM
 48
hr
 2 1098 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 323.03 NGFR rabbit IgG(H+L) / donkey NGFR 124.26
Colo858-B1-rep2
G4
WM-115 Vemurafenib MC1568 1.0000 2.6667
uM
 48
hr
 2 1007 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 320.70 NGFR rabbit IgG(H+L) / donkey NGFR 977.00
WM115-A2-rep2
G4
WM-115 Vemurafenib MC1568 1.0000 0.1067
uM
 48
hr
 2 1314 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.34 NGFR rabbit IgG(H+L) / donkey NGFR 1125.66
WM115-B2-rep2
G4