Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 2 2340 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.42 NGFR rabbit IgG(H+L) / donkey NGFR 204.08
A375-B1-rep2
B7
COLO 858 Vemurafenib PFI-1 0.3160 0.1067
uM
 48
hr
 2 2090 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 400.42 NGFR rabbit IgG(H+L) / donkey NGFR 145.17
Colo858-B2-rep2
B7
WM-115 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 2 1357 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.24 NGFR rabbit IgG(H+L) / donkey NGFR 628.79
WM115-A2-rep2
B7
A-375 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 2 2464 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.44 NGFR rabbit IgG(H+L) / donkey NGFR 154.43
A375-A2-rep2
B7
WM-115 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 2 1186 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.71 NGFR rabbit IgG(H+L) / donkey NGFR 1098.16
WM115-B2-rep2
B7
WM-115 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 1 1493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.99 NGFR rabbit IgG(H+L) / donkey NGFR 1089.78
WM115-B2-rep1
B7
COLO 858 Vemurafenib PFI-1 0.3160 2.6667
uM
 48
hr
 1 1383 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.34 NGFR rabbit IgG(H+L) / donkey NGFR 30.41
Colo858-A2-rep1
B7
A-375 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 1 2255 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.05 NGFR rabbit IgG(H+L) / donkey NGFR 162.18
A375-A2-rep1
B7
WM-115 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 2 1302 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.78 NGFR rabbit IgG(H+L) / donkey NGFR 1035.27
WM115-B1-rep2
B7
A-375 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 1 2521 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.70 NGFR rabbit IgG(H+L) / donkey NGFR 191.24
A375-B1-rep1
B7
COLO 858 Vemurafenib PFI-1 0.3160 0.5333
uM
 48
hr
 2 1454 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.50 NGFR rabbit IgG(H+L) / donkey NGFR 102.50
Colo858-B1-rep2
B7
A-375 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 1 2527 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.19 NGFR rabbit IgG(H+L) / donkey NGFR 218.09
A375-B2-rep1
B7
WM-115 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 1 1121 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.12 NGFR rabbit IgG(H+L) / donkey NGFR 999.98
WM115-B1-rep1
B7
A-375 Vemurafenib UNC669 1.0000 0.1067
uM
 48
hr
 2 2020 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.07 NGFR rabbit IgG(H+L) / donkey NGFR 160.38
A375-B2-rep2
B8
COLO 858 Vemurafenib UNC669 0.3160 0.1067
uM
 48
hr
 2 1966 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 419.20 NGFR rabbit IgG(H+L) / donkey NGFR 177.46
Colo858-B2-rep2
B8
COLO 858 Vemurafenib UNC669 0.3160 2.6667
uM
 48
hr
 2 1750 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 342.24 NGFR rabbit IgG(H+L) / donkey NGFR 153.77
Colo858-A2-rep2
B8
COLO 858 Vemurafenib UNC669 0.3160 0.5333
uM
 48
hr
 1 1503 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 309.39 NGFR rabbit IgG(H+L) / donkey NGFR 152.71
Colo858-B1-rep1
B8
COLO 858 Vemurafenib UNC669 0.3160 0.5333
uM
 48
hr
 2 1498 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 327.22 NGFR rabbit IgG(H+L) / donkey NGFR 143.43
Colo858-B1-rep2
B8
A-375 Vemurafenib UNC669 1.0000 0.5333
uM
 48
hr
 2 2288 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.78 NGFR rabbit IgG(H+L) / donkey NGFR 184.93
A375-B1-rep2
B8
WM-115 Vemurafenib UNC669 1.0000 2.6667
uM
 48
hr
 1 1162 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.02 NGFR rabbit IgG(H+L) / donkey NGFR 1039.91
WM115-A2-rep1
B8
COLO 858 Vemurafenib UNC669 0.3160 0.1067
uM
 48
hr
 1 1631 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.51 NGFR rabbit IgG(H+L) / donkey NGFR 162.38
Colo858-B2-rep1
B8
WM-115 Vemurafenib UNC669 1.0000 2.6667
uM
 48
hr
 2 1434 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.53 NGFR rabbit IgG(H+L) / donkey NGFR 1073.23
WM115-A2-rep2
B8
COLO 858 Vemurafenib UNC669 0.3160 2.6667
uM
 48
hr
 1 2097 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.07 NGFR rabbit IgG(H+L) / donkey NGFR 157.23
Colo858-A2-rep1
B8
A-375 Vemurafenib UNC669 1.0000 2.6667
uM
 48
hr
 2 2373 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 210.68 NGFR rabbit IgG(H+L) / donkey NGFR 209.48
A375-A2-rep2
B8
WM-115 Vemurafenib UNC669 1.0000 0.1067
uM
 48
hr
 1 1446 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.33 NGFR rabbit IgG(H+L) / donkey NGFR 1076.76
WM115-B2-rep1
B8