Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib GSK-J4 0.3160 0.1067
uM
 48
hr
 1 3271 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 334.17 NGFR rabbit IgG(H+L) / donkey NGFR 153.69
Colo858-B2-rep1
D6
A-375 Vemurafenib GSK-J2 1.0000 0.5333
uM
 48
hr
 1 3274 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.25 NGFR rabbit IgG(H+L) / donkey NGFR 219.64
A375-B1-rep1
C6
A-375 Vemurafenib Daminozide 1.0000 0.1067
uM
 48
hr
 1 3275 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.42 NGFR rabbit IgG(H+L) / donkey NGFR 211.84
A375-B2-rep1
E6
A-375 Vemurafenib GSK-J2 1.0000 2.6667
uM
 48
hr
 2 3289 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.33 NGFR rabbit IgG(H+L) / donkey NGFR 210.26
A375-A2-rep2
C6
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 0.1067
uM
 48
hr
 2 3297 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 322.94 NGFR rabbit IgG(H+L) / donkey NGFR 169.39
Colo858-B2-rep2
E8
A-375 Vemurafenib Entinostat 1.0000 2.6667
uM
 48
hr
 2 3308 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 589.21 NGFR rabbit IgG(H+L) / donkey NGFR 306.13
A375-A2-rep2
D4
A-375 Vemurafenib Entinostat 1.0000 0.5333
uM
 48
hr
 1 3313 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 374.52 NGFR rabbit IgG(H+L) / donkey NGFR 366.22
A375-B1-rep1
D4
A-375 Vemurafenib Entinostat 1.0000 0.1067
uM
 48
hr
 1 3329 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 267.58 NGFR rabbit IgG(H+L) / donkey NGFR 296.57
A375-B2-rep1
D4
COLO 858 Vemurafenib (-)-JQ1 0.3160 0.1067
uM
 48
hr
 2 3368 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 384.19 NGFR rabbit IgG(H+L) / donkey NGFR 168.62
Colo858-B2-rep2
D7
A-375 Vemurafenib (-)-JQ1 1.0000 0.5333
uM
 48
hr
 2 3373 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.27 NGFR rabbit IgG(H+L) / donkey NGFR 195.44
A375-B1-rep2
D7
A-375 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 2 3376 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 267.71 NGFR rabbit IgG(H+L) / donkey NGFR 465.75
A375-B2-rep2
E4
A-375 Vemurafenib AGK2 1.0000 0.1067
uM
 48
hr
 1 3383 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 234.29 NGFR rabbit IgG(H+L) / donkey NGFR 181.56
A375-B2-rep1
D5
A-375 Vemurafenib GSK-J4 1.0000 0.5333
uM
 48
hr
 1 3385 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.70 NGFR rabbit IgG(H+L) / donkey NGFR 193.11
A375-B1-rep1
D6
A-375 Vemurafenib GSK-J4 1.0000 0.1067
uM
 48
hr
 1 3386 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.57 NGFR rabbit IgG(H+L) / donkey NGFR 228.63
A375-B2-rep1
D6
COLO 858 Vemurafenib (-)-JQ1 0.3160 0.5333
uM
 48
hr
 2 3387 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.83 NGFR rabbit IgG(H+L) / donkey NGFR 150.04
Colo858-B1-rep2
D7
COLO 858 Vemurafenib GSK-J4 0.3160 0.1067
uM
 48
hr
 2 3387 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 368.22 NGFR rabbit IgG(H+L) / donkey NGFR 158.47
Colo858-B2-rep2
D6
COLO 858 Vemurafenib (-)-JQ1 0.3160 0.1067
uM
 48
hr
 1 3394 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.83 NGFR rabbit IgG(H+L) / donkey NGFR 168.87
Colo858-B2-rep1
D7
A-375 Vemurafenib (-)-JQ1 1.0000 2.6667
uM
 48
hr
 2 3409 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.33 NGFR rabbit IgG(H+L) / donkey NGFR 293.21
A375-A2-rep2
D7
COLO 858 Vemurafenib IOX2 0.3160 2.6667
uM
 48
hr
 1 3424 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 351.29 NGFR rabbit IgG(H+L) / donkey NGFR 150.84
Colo858-A2-rep1
D8
COLO 858 Vemurafenib Daminozide 0.3160 2.6667
uM
 48
hr
 1 3426 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 399.44 NGFR rabbit IgG(H+L) / donkey NGFR 145.60
Colo858-A2-rep1
E6
COLO 858 Vemurafenib Entinostat 0.3160 0.1067
uM
 48
hr
 2 3440 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 282.63 NGFR rabbit IgG(H+L) / donkey NGFR 158.24
Colo858-B2-rep2
D4
A-375 Vemurafenib GSK-J4 1.0000 0.5333
uM
 48
hr
 2 3446 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.35 NGFR rabbit IgG(H+L) / donkey NGFR 187.41
A375-B1-rep2
D6
A-375 Vemurafenib Entinostat 1.0000 0.5333
uM
 48
hr
 2 3686 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.97 NGFR rabbit IgG(H+L) / donkey NGFR 328.37
A375-B1-rep2
D4