Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 2 777 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.60 NGFR rabbit IgG(H+L) / donkey NGFR 527.85
WM115-A2-rep2
C7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 779 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.47 NGFR rabbit IgG(H+L) / donkey NGFR 983.28
WM115-B1-rep2
H4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 780 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 234.33 NGFR rabbit IgG(H+L) / donkey NGFR 134.94
Colo858-A2-rep2
H9
WM-115 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 1 784 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 114.81 NGFR rabbit IgG(H+L) / donkey NGFR 370.58
WM115-A2-rep1
F4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 787 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.73 NGFR rabbit IgG(H+L) / donkey NGFR 136.82
Colo858-B1-rep2
H2
COLO 858 Vemurafenib UNC0638 0.3160 2.6667
uM
 48
hr
 2 793 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 188.46 NGFR rabbit IgG(H+L) / donkey NGFR 153.42
Colo858-A2-rep2
G5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 793 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 260.38 NGFR rabbit IgG(H+L) / donkey NGFR 122.78
Colo858-A2-rep1
H8
COLO 858 Vemurafenib Belinostat 0.3160 0.1067
uM
 48
hr
 2 796 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.45 NGFR rabbit IgG(H+L) / donkey NGFR 129.48
Colo858-B2-rep2
C4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 797 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.09 NGFR rabbit IgG(H+L) / donkey NGFR 127.30
Colo858-A2-rep1
H6
COLO 858 Vemurafenib Belinostat 0.3160 0.1067
uM
 48
hr
 1 798 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 187.52 NGFR rabbit IgG(H+L) / donkey NGFR 66.34
Colo858-B2-rep1
C4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 801 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.52 NGFR rabbit IgG(H+L) / donkey NGFR 119.13
Colo858-A2-rep1
H11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 801 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.66 NGFR rabbit IgG(H+L) / donkey NGFR 159.65
Colo858-B1-rep2
H7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 804 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 238.63 NGFR rabbit IgG(H+L) / donkey NGFR 145.41
Colo858-B1-rep2
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 808 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.65 NGFR rabbit IgG(H+L) / donkey NGFR 855.37
WM115-A2-rep2
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 808 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.62 NGFR rabbit IgG(H+L) / donkey NGFR 1052.15
WM115-B2-rep1
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 810 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.85 NGFR rabbit IgG(H+L) / donkey NGFR 859.67
WM115-A2-rep2
H7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 811 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.76 NGFR rabbit IgG(H+L) / donkey NGFR 129.39
Colo858-A2-rep2
A4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.71 NGFR rabbit IgG(H+L) / donkey NGFR 826.75
WM115-A2-rep2
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.80 NGFR rabbit IgG(H+L) / donkey NGFR 893.83
WM115-A2-rep2
H6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.56 NGFR rabbit IgG(H+L) / donkey NGFR 951.83
WM115-B1-rep2
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.73 NGFR rabbit IgG(H+L) / donkey NGFR 976.81
WM115-B1-rep2
H2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 818 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 270.37 NGFR rabbit IgG(H+L) / donkey NGFR 132.19
Colo858-A2-rep2
H5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 821 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.47 NGFR rabbit IgG(H+L) / donkey NGFR 902.73
WM115-B1-rep2
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 821 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.01 NGFR rabbit IgG(H+L) / donkey NGFR 137.66
Colo858-B2-rep1
H5
A-375 Vemurafenib BIX-01294 1.0000 2.6667
uM
 48
hr
 1 825 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.68 NGFR rabbit IgG(H+L) / donkey NGFR 33.17
A375-A2-rep1
F5