Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 2 1158 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.88 NGFR rabbit IgG(H+L) / donkey NGFR 1182.56
WM115-B2-rep2
B6
WM-115 Vemurafenib Resveratrol 1.0000 0.1067
uM
 48
hr
 1 1377 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.59 NGFR rabbit IgG(H+L) / donkey NGFR 1187.81
WM115-B2-rep1
E5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1409 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.89 NGFR rabbit IgG(H+L) / donkey NGFR 1192.22
WM115-B2-rep1
A10
WM-115 Vemurafenib GSK-J4 1.0000 0.1067
uM
 48
hr
 2 1662 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.28 NGFR rabbit IgG(H+L) / donkey NGFR 1193.23
WM115-B2-rep2
D6
WM-115 Vemurafenib Daminozide 1.0000 0.1067
uM
 48
hr
 1 1545 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.77 NGFR rabbit IgG(H+L) / donkey NGFR 1194.58
WM115-B2-rep1
E6
WM-115 Vemurafenib GSK-J4 1.0000 0.5333
uM
 48
hr
 2 1614 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.78 NGFR rabbit IgG(H+L) / donkey NGFR 1200.46
WM115-B1-rep2
D6
WM-115 Vemurafenib (-)-JQ1 1.0000 0.5333
uM
 48
hr
 1 1613 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.43 NGFR rabbit IgG(H+L) / donkey NGFR 1200.88
WM115-B1-rep1
D7
WM-115 Vemurafenib CX-5461 1.0000 0.1067
uM
 48
hr
 1 1311 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.99 NGFR rabbit IgG(H+L) / donkey NGFR 1203.80
WM115-B2-rep1
F8
WM-115 Vemurafenib UNC1215 1.0000 0.5333
uM
 48
hr
 2 1477 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.54 NGFR rabbit IgG(H+L) / donkey NGFR 1208.27
WM115-B1-rep2
C8
WM-115 Vemurafenib GSK-J4 1.0000 0.1067
uM
 48
hr
 1 1589 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.26 NGFR rabbit IgG(H+L) / donkey NGFR 1214.14
WM115-B2-rep1
D6
WM-115 Vemurafenib CX-5461 1.0000 0.1067
uM
 48
hr
 2 1332 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.12 NGFR rabbit IgG(H+L) / donkey NGFR 1219.65
WM115-B2-rep2
F8
WM-115 Vemurafenib UNC0638 1.0000 0.1067
uM
 48
hr
 1 1111 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.67 NGFR rabbit IgG(H+L) / donkey NGFR 1220.72
WM115-B2-rep1
G5
WM-115 Vemurafenib (-)-JQ1 1.0000 0.5333
uM
 48
hr
 2 1595 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.52 NGFR rabbit IgG(H+L) / donkey NGFR 1222.46
WM115-B1-rep2
D7
WM-115 Vemurafenib AGK2 1.0000 0.1067
uM
 48
hr
 2 1468 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.46 NGFR rabbit IgG(H+L) / donkey NGFR 1223.13
WM115-B2-rep2
D5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1307 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.08 NGFR rabbit IgG(H+L) / donkey NGFR 1224.07
WM115-B1-rep2
A10
WM-115 Vemurafenib Ischemin 1.0000 0.1067
uM
 48
hr
 2 1341 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.71 NGFR rabbit IgG(H+L) / donkey NGFR 1230.55
WM115-B2-rep2
G7
WM-115 Vemurafenib UNC0638 1.0000 0.1067
uM
 48
hr
 2 1283 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.28 NGFR rabbit IgG(H+L) / donkey NGFR 1261.16
WM115-B2-rep2
G5
WM-115 Vemurafenib AGK2 1.0000 0.1067
uM
 48
hr
 1 1420 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.59 NGFR rabbit IgG(H+L) / donkey NGFR 1264.14
WM115-B2-rep1
D5
WM-115 Vemurafenib UNC669 1.0000 0.5333
uM
 48
hr
 2 1455 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.47 NGFR rabbit IgG(H+L) / donkey NGFR 1276.18
WM115-B1-rep2
B8
WM-115 Vemurafenib Entinostat 1.0000 0.1067
uM
 48
hr
 1 1701 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.88 NGFR rabbit IgG(H+L) / donkey NGFR 1358.88
WM115-B2-rep1
D4
WM-115 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 1 1586 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.99 NGFR rabbit IgG(H+L) / donkey NGFR 1412.07
WM115-B2-rep1
E4
WM-115 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 2 1572 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.44 NGFR rabbit IgG(H+L) / donkey NGFR 1493.64
WM115-B2-rep2
E4
WM-115 Vemurafenib Entinostat 1.0000 0.1067
uM
 48
hr
 2 1807 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.38 NGFR rabbit IgG(H+L) / donkey NGFR 1494.32
WM115-B2-rep2
D4