Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1235 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.84 NGFR rabbit IgG(H+L) / donkey NGFR 135.45
A375-B1-rep1
H5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 945 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 235.64 NGFR rabbit IgG(H+L) / donkey NGFR 135.45
Colo858-B1-rep2
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1065 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.49 NGFR rabbit IgG(H+L) / donkey NGFR 135.54
Colo858-B2-rep1
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1024 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.50 NGFR rabbit IgG(H+L) / donkey NGFR 135.78
Colo858-A2-rep1
A3
COLO 858 Vemurafenib Decitabine 0.3160 2.6667
uM
 48
hr
 2 1525 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 271.76 NGFR rabbit IgG(H+L) / donkey NGFR 135.82
Colo858-A2-rep2
F2
COLO 858 Vemurafenib Garcinol 0.3160 0.5333
uM
 48
hr
 1 2044 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 287.68 NGFR rabbit IgG(H+L) / donkey NGFR 135.93
Colo858-B1-rep1
F3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 880 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 236.58 NGFR rabbit IgG(H+L) / donkey NGFR 135.95
Colo858-B2-rep1
H11
A-375 Vemurafenib Anacardic acid 1.0000 0.5333
uM
 48
hr
 1 1888 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.64 NGFR rabbit IgG(H+L) / donkey NGFR 136.19
A375-B1-rep1
G3
COLO 858 Vemurafenib Selisistat 0.3160 0.1067
uM
 48
hr
 1 2270 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.67 NGFR rabbit IgG(H+L) / donkey NGFR 136.25
Colo858-B2-rep1
C5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1335 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.51 NGFR rabbit IgG(H+L) / donkey NGFR 136.29
Colo858-B1-rep1
H2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 928 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 297.12 NGFR rabbit IgG(H+L) / donkey NGFR 136.39
Colo858-B2-rep1
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1350 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.28 NGFR rabbit IgG(H+L) / donkey NGFR 136.44
Colo858-B1-rep1
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1099 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.81 NGFR rabbit IgG(H+L) / donkey NGFR 136.50
A375-A2-rep1
H8
A-375 Vemurafenib CX-5461 1.0000 0.5333
uM
 48
hr
 2 1227 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.06 NGFR rabbit IgG(H+L) / donkey NGFR 136.57
A375-B1-rep2
F8
COLO 858 Vemurafenib GSK-J2 0.3160 0.5333
uM
 48
hr
 1 2483 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 312.20 NGFR rabbit IgG(H+L) / donkey NGFR 136.57
Colo858-B1-rep1
C6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1047 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.05 NGFR rabbit IgG(H+L) / donkey NGFR 136.62
Colo858-B1-rep2
H11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 787 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.73 NGFR rabbit IgG(H+L) / donkey NGFR 136.82
Colo858-B1-rep2
H2
COLO 858 Vemurafenib JW55 0.3160 0.5333
uM
 48
hr
 2 2472 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 267.42 NGFR rabbit IgG(H+L) / donkey NGFR 137.02
Colo858-B1-rep2
D3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1209 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.38 NGFR rabbit IgG(H+L) / donkey NGFR 137.13
Colo858-B2-rep1
A6
A-375 Vemurafenib RG-108 1.0000 0.5333
uM
 48
hr
 1 1759 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.66 NGFR rabbit IgG(H+L) / donkey NGFR 137.19
A375-B1-rep1
G2
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 0.5333
uM
 48
hr
 2 2802 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 324.58 NGFR rabbit IgG(H+L) / donkey NGFR 137.24
Colo858-B1-rep2
E8
COLO 858 Vemurafenib Ischemin 0.3160 0.1067
uM
 48
hr
 2 1683 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 304.71 NGFR rabbit IgG(H+L) / donkey NGFR 137.29
Colo858-B2-rep2
G7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1514 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.99 NGFR rabbit IgG(H+L) / donkey NGFR 137.41
A375-B2-rep2
H9
A-375 Vemurafenib Azacitidine 1.0000 0.1067
uM
 48
hr
 2 2202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.55 NGFR rabbit IgG(H+L) / donkey NGFR 137.51
A375-B2-rep2
E2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1248 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.32 NGFR rabbit IgG(H+L) / donkey NGFR 137.61
A375-B1-rep2
H9