Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib AGK2 1.0000 2.6667
uM
 48
hr
 1 678 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 394.51 NGFR rabbit IgG(H+L) / donkey NGFR 132.96
A375-A2-rep1
D5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1359 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 394.62 NGFR rabbit IgG(H+L) / donkey NGFR 149.61
Colo858-B2-rep2
A9
A-375 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 1 279 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 395.49 NGFR rabbit IgG(H+L) / donkey NGFR 21.95
A375-A2-rep1
C4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 972 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 397.01 NGFR rabbit IgG(H+L) / donkey NGFR 132.49
Colo858-A2-rep2
A8
COLO 858 Vemurafenib Daminozide 0.3160 2.6667
uM
 48
hr
 1 3426 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 399.44 NGFR rabbit IgG(H+L) / donkey NGFR 145.60
Colo858-A2-rep1
E6
COLO 858 Vemurafenib PFI-1 0.3160 0.1067
uM
 48
hr
 2 2090 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 400.42 NGFR rabbit IgG(H+L) / donkey NGFR 145.17
Colo858-B2-rep2
B7
COLO 858 Vemurafenib IOX2 0.3160 0.1067
uM
 48
hr
 2 3195 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 403.34 NGFR rabbit IgG(H+L) / donkey NGFR 186.02
Colo858-B2-rep2
D8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1020 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 404.61 NGFR rabbit IgG(H+L) / donkey NGFR 144.69
Colo858-B2-rep2
A8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1117 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 406.39 NGFR rabbit IgG(H+L) / donkey NGFR 145.48
Colo858-A2-rep2
A5
COLO 858 Vemurafenib GSK-J1 0.3160 0.1067
uM
 48
hr
 2 1949 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 408.18 NGFR rabbit IgG(H+L) / donkey NGFR 166.40
Colo858-B2-rep2
B6
A-375 Vemurafenib Vorinostat 1.0000 0.5333
uM
 48
hr
 1 2811 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.35 NGFR rabbit IgG(H+L) / donkey NGFR 111.10
A375-B1-rep1
D2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1070 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 412.54 NGFR rabbit IgG(H+L) / donkey NGFR 139.52
Colo858-B2-rep2
A3
COLO 858 Vemurafenib UNC669 0.3160 0.1067
uM
 48
hr
 2 1966 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 419.20 NGFR rabbit IgG(H+L) / donkey NGFR 177.46
Colo858-B2-rep2
B8
A-375 Vemurafenib AGK2 1.0000 2.6667
uM
 48
hr
 2 1329 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 425.48 NGFR rabbit IgG(H+L) / donkey NGFR 176.32
A375-A2-rep2
D5
A-375 Vemurafenib Pracinostat 1.0000 0.5333
uM
 48
hr
 1 1766 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 425.92 NGFR rabbit IgG(H+L) / donkey NGFR 51.29
A375-B1-rep1
F4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 949 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 432.52 NGFR rabbit IgG(H+L) / donkey NGFR 134.87
Colo858-B2-rep2
A6
A-375 Vemurafenib Vorinostat 1.0000 0.5333
uM
 48
hr
 2 2869 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 443.59 NGFR rabbit IgG(H+L) / donkey NGFR 119.80
A375-B1-rep2
D2
A-375 Vemurafenib Entinostat 1.0000 2.6667
uM
 48
hr
 1 2292 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.09 NGFR rabbit IgG(H+L) / donkey NGFR 264.91
A375-A2-rep1
D4
A-375 Vemurafenib MC1568 1.0000 2.6667
uM
 48
hr
 2 2431 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 484.57 NGFR rabbit IgG(H+L) / donkey NGFR 158.89
A375-A2-rep2
G4
A-375 Vemurafenib Pracinostat 1.0000 0.1067
uM
 48
hr
 1 2816 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 487.81 NGFR rabbit IgG(H+L) / donkey NGFR 126.79
A375-B2-rep1
F4
A-375 Vemurafenib Pracinostat 1.0000 0.5333
uM
 48
hr
 2 2095 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 492.96 NGFR rabbit IgG(H+L) / donkey NGFR 61.23
A375-B1-rep2
F4
A-375 Vemurafenib Pracinostat 1.0000 0.1067
uM
 48
hr
 2 3139 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 547.64 NGFR rabbit IgG(H+L) / donkey NGFR 127.96
A375-B2-rep2
F4
A-375 Vemurafenib Entinostat 1.0000 2.6667
uM
 48
hr
 2 3308 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 589.21 NGFR rabbit IgG(H+L) / donkey NGFR 306.13
A375-A2-rep2
D4