Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib RG-108 0.3160 0.1067
uM
 48
hr
 1 1249 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 276.06 NGFR rabbit IgG(H+L) / donkey NGFR 152.83
Colo858-B2-rep1
G2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 673 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 276.26 NGFR rabbit IgG(H+L) / donkey NGFR 130.08
Colo858-A2-rep1
H5
COLO 858 Vemurafenib Rucaparib 0.3160 0.5333
uM
 48
hr
 2 2053 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 276.51 NGFR rabbit IgG(H+L) / donkey NGFR 162.63
Colo858-B1-rep2
C3
COLO 858 Vemurafenib Rocilinostat 0.3160 0.5333
uM
 48
hr
 1 1567 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.32 NGFR rabbit IgG(H+L) / donkey NGFR 207.37
Colo858-B1-rep1
B5
A-375 Vemurafenib Rocilinostat 1.0000 2.6667
uM
 48
hr
 2 2940 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.54 NGFR rabbit IgG(H+L) / donkey NGFR 76.23
A375-A2-rep2
B5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 920 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.58 NGFR rabbit IgG(H+L) / donkey NGFR 140.83
Colo858-A2-rep2
H3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 683 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.61 NGFR rabbit IgG(H+L) / donkey NGFR 110.87
Colo858-A2-rep1
H4
A-375 Vemurafenib Vorinostat 1.0000 0.1067
uM
 48
hr
 2 2959 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.98 NGFR rabbit IgG(H+L) / donkey NGFR 129.76
A375-B2-rep2
D2
COLO 858 Vemurafenib Veliparib 0.3160 0.1067
uM
 48
hr
 1 1792 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.98 NGFR rabbit IgG(H+L) / donkey NGFR 153.52
Colo858-B2-rep1
C2
COLO 858 Vemurafenib PFI-1 0.3160 0.5333
uM
 48
hr
 1 1513 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.22 NGFR rabbit IgG(H+L) / donkey NGFR 84.85
Colo858-B1-rep1
B7
COLO 858 Vemurafenib CTB 0.3160 0.1067
uM
 48
hr
 1 1399 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.66 NGFR rabbit IgG(H+L) / donkey NGFR 181.20
Colo858-B2-rep1
B4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 922 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.87 NGFR rabbit IgG(H+L) / donkey NGFR 126.84
Colo858-A2-rep2
H8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 709 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.92 NGFR rabbit IgG(H+L) / donkey NGFR 116.26
Colo858-B2-rep1
H3
A-375 Vemurafenib Rocilinostat 1.0000 2.6667
uM
 48
hr
 1 2722 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 279.18 NGFR rabbit IgG(H+L) / donkey NGFR 95.39
A375-A2-rep1
B5
COLO 858 Vemurafenib AGK2 0.3160 0.1067
uM
 48
hr
 1 2399 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 279.22 NGFR rabbit IgG(H+L) / donkey NGFR 124.75
Colo858-B2-rep1
D5
COLO 858 Vemurafenib Garcinol 0.3160 2.6667
uM
 48
hr
 2 1133 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 279.50 NGFR rabbit IgG(H+L) / donkey NGFR 140.01
Colo858-A2-rep2
F3
COLO 858 Vemurafenib Methylstat 0.3160 0.1067
uM
 48
hr
 1 2248 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 279.63 NGFR rabbit IgG(H+L) / donkey NGFR 146.80
Colo858-B2-rep1
F6
COLO 858 Vemurafenib C646 0.3160 0.5333
uM
 48
hr
 1 2531 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.51 NGFR rabbit IgG(H+L) / donkey NGFR 145.18
Colo858-B1-rep1
E3
A-375 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 1 2929 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.55 NGFR rabbit IgG(H+L) / donkey NGFR 517.04
A375-B2-rep1
E4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1037 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.57 NGFR rabbit IgG(H+L) / donkey NGFR 127.15
Colo858-B1-rep1
H6
COLO 858 Vemurafenib JW55 0.3160 0.1067
uM
 48
hr
 1 2407 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.93 NGFR rabbit IgG(H+L) / donkey NGFR 154.25
Colo858-B2-rep1
D3
COLO 858 Vemurafenib Garcinol 0.3160 0.5333
uM
 48
hr
 2 1674 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 281.10 NGFR rabbit IgG(H+L) / donkey NGFR 142.39
Colo858-B1-rep2
F3
COLO 858 Vemurafenib Azacitidine 0.3160 0.5333
uM
 48
hr
 2 1199 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 281.80 NGFR rabbit IgG(H+L) / donkey NGFR 95.43
Colo858-B1-rep2
E2
COLO 858 Vemurafenib MC1568 0.3160 0.1067
uM
 48
hr
 1 1269 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 281.92 NGFR rabbit IgG(H+L) / donkey NGFR 115.06
Colo858-B2-rep1
G4
A-375 Vemurafenib Rocilinostat 1.0000 0.5333
uM
 48
hr
 1 3162 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 282.48 NGFR rabbit IgG(H+L) / donkey NGFR 131.05
A375-B1-rep1
B5