Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib CX-5461 1.0000 2.6667
uM
 48
hr
 1 1088 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.79 NGFR rabbit IgG(H+L) / donkey NGFR 1051.81
WM115-A2-rep1
F8
WM-115 Vemurafenib C646 1.0000 2.6667
uM
 48
hr
 2 1111 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.86 NGFR rabbit IgG(H+L) / donkey NGFR 957.59
WM115-A2-rep2
E3
WM-115 Vemurafenib I-BET151 1.0000 2.6667
uM
 48
hr
 2 768 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.34 NGFR rabbit IgG(H+L) / donkey NGFR 479.27
WM115-A2-rep2
F7
WM-115 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 2 1572 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.44 NGFR rabbit IgG(H+L) / donkey NGFR 1493.64
WM115-B2-rep2
E4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 808 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.65 NGFR rabbit IgG(H+L) / donkey NGFR 855.37
WM115-A2-rep2
H4
WM-115 Vemurafenib Ischemin 1.0000 0.1067
uM
 48
hr
 2 1341 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.71 NGFR rabbit IgG(H+L) / donkey NGFR 1230.55
WM115-B2-rep2
G7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.80 NGFR rabbit IgG(H+L) / donkey NGFR 893.83
WM115-A2-rep2
H6
WM-115 Vemurafenib CX-5461 1.0000 0.5333
uM
 48
hr
 1 1376 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.03 NGFR rabbit IgG(H+L) / donkey NGFR 1089.00
WM115-B1-rep1
F8
WM-115 Vemurafenib I-BET 1.0000 2.6667
uM
 48
hr
 2 1147 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.03 NGFR rabbit IgG(H+L) / donkey NGFR 490.84
WM115-A2-rep2
E7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1049 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.43 NGFR rabbit IgG(H+L) / donkey NGFR 1010.51
WM115-B2-rep2
A5
A-375 Vemurafenib CX-5461 1.0000 0.1067
uM
 48
hr
 2 1802 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.80 NGFR rabbit IgG(H+L) / donkey NGFR 134.98
A375-B2-rep2
F8
WM-115 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 1 521 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.85 NGFR rabbit IgG(H+L) / donkey NGFR 452.89
WM115-A2-rep1
C7
WM-115 Vemurafenib Entinostat 1.0000 0.5333
uM
 48
hr
 2 1744 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.89 NGFR rabbit IgG(H+L) / donkey NGFR 1128.25
WM115-B1-rep2
D4
WM-115 Vemurafenib Garcinol 1.0000 0.5333
uM
 48
hr
 2 1114 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.06 NGFR rabbit IgG(H+L) / donkey NGFR 1016.89
WM115-B1-rep2
F3
WM-115 Vemurafenib Resveratrol 1.0000 2.6667
uM
 48
hr
 1 1297 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.13 NGFR rabbit IgG(H+L) / donkey NGFR 1028.89
WM115-A2-rep1
E5
A-375 Vemurafenib (+)-JQ1 1.0000 0.1067
uM
 48
hr
 2 2498 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.23 NGFR rabbit IgG(H+L) / donkey NGFR 95.78
A375-B2-rep2
C7
WM-115 Vemurafenib PFI-1 1.0000 2.6667
uM
 48
hr
 2 1357 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.24 NGFR rabbit IgG(H+L) / donkey NGFR 628.79
WM115-A2-rep2
B7
WM-115 Vemurafenib I-BET 1.0000 0.5333
uM
 48
hr
 2 1311 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.35 NGFR rabbit IgG(H+L) / donkey NGFR 741.26
WM115-B1-rep2
E7
WM-115 Vemurafenib Pracinostat 1.0000 0.1067
uM
 48
hr
 1 1425 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.48 NGFR rabbit IgG(H+L) / donkey NGFR 911.70
WM115-B2-rep1
F4
A-375 Vemurafenib (+)-JQ1 1.0000 0.5333
uM
 48
hr
 1 2279 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.52 NGFR rabbit IgG(H+L) / donkey NGFR 79.90
A375-B1-rep1
C7
WM-115 Vemurafenib Tranylcypromine 1.0000 2.6667
uM
 48
hr
 2 906 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.81 NGFR rabbit IgG(H+L) / donkey NGFR 1167.67
WM115-A2-rep2
G6
WM-115 Vemurafenib Methylstat 1.0000 0.5333
uM
 48
hr
 1 1289 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.93 NGFR rabbit IgG(H+L) / donkey NGFR 1114.18
WM115-B1-rep1
F6
A-375 Vemurafenib CX-5461 1.0000 0.5333
uM
 48
hr
 2 1227 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.06 NGFR rabbit IgG(H+L) / donkey NGFR 136.57
A375-B1-rep2
F8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 966 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.08 NGFR rabbit IgG(H+L) / donkey NGFR 1010.70
WM115-B1-rep1
H3
WM-115 Vemurafenib Rocilinostat 1.0000 0.5333
uM
 48
hr
 1 1305 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.30 NGFR rabbit IgG(H+L) / donkey NGFR 965.10
WM115-B1-rep1
B5