Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1009 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 308.25 NGFR rabbit IgG(H+L) / donkey NGFR 142.85
Colo858-B1-rep1
H11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1172 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 265.99 NGFR rabbit IgG(H+L) / donkey NGFR 115.59
Colo858-B1-rep1
H10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1228 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.70 NGFR rabbit IgG(H+L) / donkey NGFR 125.18
Colo858-B1-rep1
A9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 970 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 376.63 NGFR rabbit IgG(H+L) / donkey NGFR 125.61
Colo858-B1-rep1
A8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1082 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.68 NGFR rabbit IgG(H+L) / donkey NGFR 117.87
Colo858-B1-rep1
A7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1005 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 348.84 NGFR rabbit IgG(H+L) / donkey NGFR 143.10
Colo858-B1-rep1
A6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1008 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 371.10 NGFR rabbit IgG(H+L) / donkey NGFR 120.59
Colo858-B1-rep1
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1333 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 323.49 NGFR rabbit IgG(H+L) / donkey NGFR 141.87
Colo858-B2-rep2
H10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1012 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 248.16 NGFR rabbit IgG(H+L) / donkey NGFR 135.16
Colo858-B2-rep2
H11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1281 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 283.24 NGFR rabbit IgG(H+L) / donkey NGFR 140.21
Colo858-B2-rep2
H2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1355 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 285.72 NGFR rabbit IgG(H+L) / donkey NGFR 149.36
Colo858-B2-rep2
H3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1117 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 282.92 NGFR rabbit IgG(H+L) / donkey NGFR 133.48
Colo858-B2-rep2
H4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 930 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 246.88 NGFR rabbit IgG(H+L) / donkey NGFR 124.72
Colo858-B2-rep2
H5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1071 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 252.70 NGFR rabbit IgG(H+L) / donkey NGFR 127.95
Colo858-B2-rep2
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1049 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.15 NGFR rabbit IgG(H+L) / donkey NGFR 133.13
Colo858-B2-rep2
H7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 943 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.12 NGFR rabbit IgG(H+L) / donkey NGFR 129.28
Colo858-B2-rep2
H8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 271.95 NGFR rabbit IgG(H+L) / donkey NGFR 134.54
Colo858-B2-rep2
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1093 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.58 NGFR rabbit IgG(H+L) / donkey NGFR 1088.13
WM115-A2-rep1
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1094 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.45 NGFR rabbit IgG(H+L) / donkey NGFR 1030.60
WM115-A2-rep1
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1314 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.02 NGFR rabbit IgG(H+L) / donkey NGFR 966.82
WM115-A2-rep1
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1054 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.53 NGFR rabbit IgG(H+L) / donkey NGFR 926.14
WM115-A2-rep1
A3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1158 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.15 NGFR rabbit IgG(H+L) / donkey NGFR 1015.40
WM115-A2-rep1
A4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 929 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.87 NGFR rabbit IgG(H+L) / donkey NGFR 964.82
WM115-A2-rep1
A5