Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 764 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 266.14 NGFR rabbit IgG(H+L) / donkey NGFR 145.66
Colo858-B1-rep2
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 685 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 282.57 NGFR rabbit IgG(H+L) / donkey NGFR 147.29
Colo858-B1-rep2
H5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 804 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 238.63 NGFR rabbit IgG(H+L) / donkey NGFR 145.41
Colo858-B1-rep2
H4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 610 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 248.64 NGFR rabbit IgG(H+L) / donkey NGFR 141.66
Colo858-B1-rep2
H3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 787 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.73 NGFR rabbit IgG(H+L) / donkey NGFR 136.82
Colo858-B1-rep2
H2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1047 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.05 NGFR rabbit IgG(H+L) / donkey NGFR 136.62
Colo858-B1-rep2
H11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1057 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 233.24 NGFR rabbit IgG(H+L) / donkey NGFR 123.44
Colo858-B1-rep2
H10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1238 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 334.50 NGFR rabbit IgG(H+L) / donkey NGFR 151.42
Colo858-B1-rep2
A9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 685 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 333.97 NGFR rabbit IgG(H+L) / donkey NGFR 138.64
Colo858-B1-rep2
A8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 911 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 350.80 NGFR rabbit IgG(H+L) / donkey NGFR 124.13
Colo858-B1-rep2
A7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 994 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 355.47 NGFR rabbit IgG(H+L) / donkey NGFR 129.93
Colo858-B1-rep2
A6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 856 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 298.17 NGFR rabbit IgG(H+L) / donkey NGFR 127.54
Colo858-B1-rep2
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1106 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 325.17 NGFR rabbit IgG(H+L) / donkey NGFR 144.51
Colo858-B1-rep2
A4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 915 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.91 NGFR rabbit IgG(H+L) / donkey NGFR 130.55
Colo858-B1-rep2
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 906 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.23 NGFR rabbit IgG(H+L) / donkey NGFR 108.59
Colo858-B1-rep2
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 994 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.13 NGFR rabbit IgG(H+L) / donkey NGFR 159.64
Colo858-B1-rep2
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1231 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 346.46 NGFR rabbit IgG(H+L) / donkey NGFR 151.76
Colo858-B1-rep2
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1265 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.56 NGFR rabbit IgG(H+L) / donkey NGFR 114.69
Colo858-B1-rep1
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1014 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 285.27 NGFR rabbit IgG(H+L) / donkey NGFR 120.90
Colo858-B1-rep1
H8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1122 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.07 NGFR rabbit IgG(H+L) / donkey NGFR 147.66
Colo858-B1-rep1
H7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1037 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.57 NGFR rabbit IgG(H+L) / donkey NGFR 127.15
Colo858-B1-rep1
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1132 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.65 NGFR rabbit IgG(H+L) / donkey NGFR 141.85
Colo858-B1-rep1
H5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1070 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 308.39 NGFR rabbit IgG(H+L) / donkey NGFR 124.26
Colo858-B1-rep1
H4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1171 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 341.30 NGFR rabbit IgG(H+L) / donkey NGFR 131.95
Colo858-B1-rep1
H3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1335 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.51 NGFR rabbit IgG(H+L) / donkey NGFR 136.29
Colo858-B1-rep1
H2