Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1672 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.21 NGFR rabbit IgG(H+L) / donkey NGFR 175.60
A375-A2-rep1
A9
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1857 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.14 NGFR rabbit IgG(H+L) / donkey NGFR 189.83
A375-A2-rep1
A8
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.41 NGFR rabbit IgG(H+L) / donkey NGFR 186.71
A375-A2-rep1
A7
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2263 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 182.94 NGFR rabbit IgG(H+L) / donkey NGFR 198.78
A375-A2-rep1
A6
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2252 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 227.51 NGFR rabbit IgG(H+L) / donkey NGFR 199.22
A375-A2-rep1
A5
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2171 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 208.08 NGFR rabbit IgG(H+L) / donkey NGFR 204.03
A375-A2-rep1
A4
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2008 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.44 NGFR rabbit IgG(H+L) / donkey NGFR 205.73
A375-A2-rep1
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2214 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.79 NGFR rabbit IgG(H+L) / donkey NGFR 205.29
A375-A2-rep1
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1506 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.95 NGFR rabbit IgG(H+L) / donkey NGFR 162.28
A375-A2-rep1
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1150 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.75 NGFR rabbit IgG(H+L) / donkey NGFR 1097.89
WM115-B2-rep2
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1002 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.83 NGFR rabbit IgG(H+L) / donkey NGFR 994.93
WM115-B2-rep2
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1108 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.07 NGFR rabbit IgG(H+L) / donkey NGFR 1162.21
WM115-B2-rep2
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1162 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.28 NGFR rabbit IgG(H+L) / donkey NGFR 1154.34
WM115-B2-rep2
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1118 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.53 NGFR rabbit IgG(H+L) / donkey NGFR 1078.75
WM115-B2-rep2
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1141 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.91 NGFR rabbit IgG(H+L) / donkey NGFR 1132.64
WM115-B2-rep2
H5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.32 NGFR rabbit IgG(H+L) / donkey NGFR 1076.20
WM115-B2-rep2
H6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1059 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.16 NGFR rabbit IgG(H+L) / donkey NGFR 1102.52
WM115-B2-rep2
H7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1144 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.31 NGFR rabbit IgG(H+L) / donkey NGFR 1008.74
WM115-B2-rep2
H8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1118 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.18 NGFR rabbit IgG(H+L) / donkey NGFR 1039.92
WM115-B2-rep2
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 815 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.71 NGFR rabbit IgG(H+L) / donkey NGFR 826.75
WM115-A2-rep2
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 867 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.77 NGFR rabbit IgG(H+L) / donkey NGFR 905.23
WM115-A2-rep2
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 991 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.39 NGFR rabbit IgG(H+L) / donkey NGFR 912.83
WM115-A2-rep2
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 854 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.28 NGFR rabbit IgG(H+L) / donkey NGFR 899.54
WM115-A2-rep2
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 808 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.65 NGFR rabbit IgG(H+L) / donkey NGFR 855.37
WM115-A2-rep2
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 754 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.51 NGFR rabbit IgG(H+L) / donkey NGFR 910.39
WM115-A2-rep2
H5