Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1074 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.31 NGFR rabbit IgG(H+L) / donkey NGFR 161.00
A375-A2-rep1
H7
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 969 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.13 NGFR rabbit IgG(H+L) / donkey NGFR 146.47
A375-A2-rep1
H6
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 955 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.67 NGFR rabbit IgG(H+L) / donkey NGFR 131.81
A375-A2-rep1
H5
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1004 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.24 NGFR rabbit IgG(H+L) / donkey NGFR 122.61
A375-A2-rep1
H4
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1034 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.56 NGFR rabbit IgG(H+L) / donkey NGFR 144.52
A375-A2-rep1
H3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1206 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.77 NGFR rabbit IgG(H+L) / donkey NGFR 156.70
A375-A2-rep1
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1144 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.31 NGFR rabbit IgG(H+L) / donkey NGFR 1008.74
WM115-B2-rep2
H8
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1210 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.35 NGFR rabbit IgG(H+L) / donkey NGFR 155.16
A375-A2-rep1
H11
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1088 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.47 NGFR rabbit IgG(H+L) / donkey NGFR 155.22
A375-A2-rep1
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 883 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.08 NGFR rabbit IgG(H+L) / donkey NGFR 1102.94
WM115-B2-rep1
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 952 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.09 NGFR rabbit IgG(H+L) / donkey NGFR 915.79
WM115-B2-rep1
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 939 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.31 NGFR rabbit IgG(H+L) / donkey NGFR 946.36
WM115-B2-rep1
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 845 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.87 NGFR rabbit IgG(H+L) / donkey NGFR 1057.39
WM115-B2-rep1
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 808 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.62 NGFR rabbit IgG(H+L) / donkey NGFR 1052.15
WM115-B2-rep1
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 746 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.54 NGFR rabbit IgG(H+L) / donkey NGFR 1007.03
WM115-B2-rep1
H5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 904 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.03 NGFR rabbit IgG(H+L) / donkey NGFR 939.97
WM115-B2-rep1
H6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 861 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.45 NGFR rabbit IgG(H+L) / donkey NGFR 1013.19
WM115-B2-rep1
H7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 876 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.53 NGFR rabbit IgG(H+L) / donkey NGFR 1049.12
WM115-B2-rep1
H8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 906 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.77 NGFR rabbit IgG(H+L) / donkey NGFR 1117.05
WM115-B2-rep1
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1100 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.13 NGFR rabbit IgG(H+L) / donkey NGFR 1179.05
WM115-B2-rep2
A10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1179 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.88 NGFR rabbit IgG(H+L) / donkey NGFR 1171.65
WM115-B2-rep2
A11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1222 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.91 NGFR rabbit IgG(H+L) / donkey NGFR 1104.02
WM115-B2-rep2
A2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1434 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.89 NGFR rabbit IgG(H+L) / donkey NGFR 1166.16
WM115-B2-rep2
A3