Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib IOX2 0.3160 0.5333
uM
 48
hr
 1 2770 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.63 NGFR rabbit IgG(H+L) / donkey NGFR 134.52
Colo858-B1-rep1
D8
COLO 858 Vemurafenib Entinostat 0.3160 0.5333
uM
 48
hr
 1 2835 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 224.61 NGFR rabbit IgG(H+L) / donkey NGFR 79.92
Colo858-B1-rep1
D4
COLO 858 Vemurafenib JW55 0.3160 0.5333
uM
 48
hr
 1 2560 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 320.04 NGFR rabbit IgG(H+L) / donkey NGFR 134.51
Colo858-B1-rep1
D3
COLO 858 Vemurafenib AGK2 0.3160 0.5333
uM
 48
hr
 1 1897 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 317.36 NGFR rabbit IgG(H+L) / donkey NGFR 134.31
Colo858-B1-rep1
D5
COLO 858 Vemurafenib Azacitidine 0.3160 0.5333
uM
 48
hr
 1 1775 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.70 NGFR rabbit IgG(H+L) / donkey NGFR 98.06
Colo858-B1-rep1
E2
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.5333
uM
 48
hr
 1 1371 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 102.15 NGFR rabbit IgG(H+L) / donkey NGFR 16.33
Colo858-B1-rep1
C7
A-375 Vemurafenib Ischemin 1.0000 0.5333
uM
 48
hr
 2 1846 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 204.07 NGFR rabbit IgG(H+L) / donkey NGFR 168.74
A375-B1-rep2
G7
COLO 858 Vemurafenib UNC1215 0.3160 0.5333
uM
 48
hr
 1 2463 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 322.32 NGFR rabbit IgG(H+L) / donkey NGFR 160.13
Colo858-B1-rep1
C8
A-375 Vemurafenib Tranylcypromine 1.0000 0.5333
uM
 48
hr
 2 1765 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 166.51 NGFR rabbit IgG(H+L) / donkey NGFR 168.79
A375-B1-rep2
G6
A-375 Vemurafenib UNC0638 1.0000 0.5333
uM
 48
hr
 2 1689 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 178.69 NGFR rabbit IgG(H+L) / donkey NGFR 113.11
A375-B1-rep2
G5
COLO 858 Vemurafenib Vorinostat 0.3160 0.5333
uM
 48
hr
 1 1313 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 213.34 NGFR rabbit IgG(H+L) / donkey NGFR 151.95
Colo858-B1-rep1
D2
COLO 858 Vemurafenib C646 0.3160 0.5333
uM
 48
hr
 1 2531 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.51 NGFR rabbit IgG(H+L) / donkey NGFR 145.18
Colo858-B1-rep1
E3
A-375 Vemurafenib MC1568 1.0000 0.5333
uM
 48
hr
 2 2064 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 299.54 NGFR rabbit IgG(H+L) / donkey NGFR 157.98
A375-B1-rep2
G4
A-375 Vemurafenib Anacardic acid 1.0000 0.5333
uM
 48
hr
 2 1745 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.73 NGFR rabbit IgG(H+L) / donkey NGFR 158.90
A375-B1-rep2
G3
COLO 858 Vemurafenib Belinostat 0.3160 0.5333
uM
 48
hr
 1 394 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.28 NGFR rabbit IgG(H+L) / donkey NGFR 28.69
Colo858-B1-rep1
C4
A-375 Vemurafenib RG-108 1.0000 0.5333
uM
 48
hr
 2 1758 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.76 NGFR rabbit IgG(H+L) / donkey NGFR 147.11
A375-B1-rep2
G2
A-375 Vemurafenib CX-5461 1.0000 0.5333
uM
 48
hr
 2 1227 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.06 NGFR rabbit IgG(H+L) / donkey NGFR 136.57
A375-B1-rep2
F8
COLO 858 Vemurafenib Selisistat 0.3160 0.5333
uM
 48
hr
 1 2548 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 330.10 NGFR rabbit IgG(H+L) / donkey NGFR 118.10
Colo858-B1-rep1
C5
A-375 Vemurafenib I-BET151 1.0000 0.5333
uM
 48
hr
 2 2401 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.82 NGFR rabbit IgG(H+L) / donkey NGFR 109.14
A375-B1-rep2
F7
A-375 Vemurafenib Methylstat 1.0000 0.5333
uM
 48
hr
 2 2362 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.85 NGFR rabbit IgG(H+L) / donkey NGFR 141.45
A375-B1-rep2
F6
A-375 Vemurafenib BIX-01294 1.0000 0.5333
uM
 48
hr
 2 2173 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.24 NGFR rabbit IgG(H+L) / donkey NGFR 105.39
A375-B1-rep2
F5
A-375 Vemurafenib Pracinostat 1.0000 0.5333
uM
 48
hr
 2 2095 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 492.96 NGFR rabbit IgG(H+L) / donkey NGFR 61.23
A375-B1-rep2
F4
COLO 858 Vemurafenib GSK-J2 0.3160 0.5333
uM
 48
hr
 1 2483 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 312.20 NGFR rabbit IgG(H+L) / donkey NGFR 136.57
Colo858-B1-rep1
C6
COLO 858 Vemurafenib Mocetinostat 0.3160 0.5333
uM
 48
hr
 1 2873 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.87 NGFR rabbit IgG(H+L) / donkey NGFR 44.51
Colo858-B1-rep1
E4
COLO 858 Vemurafenib I-BET 0.3160 0.5333
uM
 48
hr
 1 2681 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.14 NGFR rabbit IgG(H+L) / donkey NGFR 33.28
Colo858-B1-rep1
E7