Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 609.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.70 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 356.06
A375-P4
F3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 986.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 219.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 678.93
Colo858-P3
F3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1053.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 226.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 692.16
Colo858-P3
F2
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 684.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.91 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 357.40
A375-P4
C2
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 725.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 207.31 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1613.01
A375-P2
D2
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1022.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 229.98 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2502.46
A375-P2
F3
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2981.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 513.45 NGFR rabbit IgG(H+L) / donkey NGFR 573.44
A375-P1
B3
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 837.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 588.81
A375-P3
F2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2269.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 298.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 129.41
Colo858-P3
B3
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 688.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.69 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 363.53
A375-P4
C3
WM-115 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 745.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.62 NGFR rabbit IgG(H+L) / donkey NGFR 1796.89
WM115-P1
F2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2543.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 325.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 151.83
Colo858-P3
E3
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1042.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 234.63 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2631.95
A375-P2
F2
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 876.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.24 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1131.61
Colo858-P2
C3
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2508.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 298.52 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 135.52
Colo858-P3
E2
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2735.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 434.01
A375-P3
E2
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1159.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 248.75 NGFR rabbit IgG(H+L) / donkey NGFR 1290.83
WM115-P1
E2
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 485.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.58 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 293.43
A375-P4
D2
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1073.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 215.52 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1115.43
Colo858-P2
C2
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1139.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 238.74 NGFR rabbit IgG(H+L) / donkey NGFR 1292.52
WM115-P1
E3
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2254.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 328.28 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4887.35
Colo858-P2
B3
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 787.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.96 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2136.36
A375-P2
C3
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 786.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 210.29 NGFR rabbit IgG(H+L) / donkey NGFR 822.61
A375-P1
C3
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 658.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.52 NGFR rabbit IgG(H+L) / donkey NGFR 784.50
A375-P1
C2
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1069.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 249.87 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2417.92
A375-P2
C2