Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 360.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.77 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 402.98
Colo858-P4
C5
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.02 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 186.62
WM115-P4
B4
WM-115 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 94.54 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 156.69
WM115-P4
B5
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 445.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 403.95
Colo858-P4
C4
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 298.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 102.91 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 161.75
WM115-P4
C4
WM-115 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 201.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 79.20 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 134.69
WM115-P4
C5
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 389.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.61 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 275.98
Colo858-P2
C5
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 663.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 168.59 NGFR rabbit IgG(H+L) / donkey NGFR 1157.13
Colo858-P1
C4
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 572.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.44 NGFR rabbit IgG(H+L) / donkey NGFR 1097.04
Colo858-P1
C5
COLO 858 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 493.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 164.54 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 388.63
Colo858-P4
B5
WM-115 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 481.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.75 NGFR rabbit IgG(H+L) / donkey NGFR 3327.77
WM115-P1
D4
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.06 NGFR rabbit IgG(H+L) / donkey NGFR 3192.24
WM115-P1
D5
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 102.23 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 158.98
WM115-P4
D5
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 465.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.80 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 405.55
Colo858-P4
B4
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.41 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 713.04
A375-P3
F4
COLO 858 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 390.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.15 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 318.20
Colo858-P2
E5
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 154.85
WM115-P4
E4
WM-115 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 286.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.41 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 183.08
WM115-P4
E5
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 236.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.87 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 347.87
A375-P4
D4
COLO 858 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 538.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 151.96 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 869.75
Colo858-P3
G5
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 430.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 981.08
Colo858-P3
G4
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 316.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 93.67 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 171.12
WM115-P4
F4
WM-115 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 94.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 151.92
WM115-P4
F5
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 758.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 202.93 NGFR rabbit IgG(H+L) / donkey NGFR 1207.94
Colo858-P1
G4
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 447.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 164.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 948.70
Colo858-P3
F5