Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 892.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.01 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 314.05
A375-P10
C6
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 737.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 204.50 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 267.04
A375-P10
C7
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1065.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 221.73 NGFR rabbit IgG(H+L) / donkey NGFR 534.00
A375-P7
F6
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1446.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 307.33 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 471.65
A375-P8
B7
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 650.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 186.68 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 81.26
A375-P8
D6
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1464.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 324.97 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 974.92
A375-P8
B6
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2022.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 242.98 NGFR rabbit IgG(H+L) / donkey NGFR 131.68
Colo858-P7
B7
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 908.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.71 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 75.37
A375-P8
C7
COLO 858 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 832.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 151.64 NGFR rabbit IgG(H+L) / donkey NGFR 667.24
Colo858-P7
D6
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 985.54 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 226.86 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 90.96
A375-P8
C6
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 534.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 299.97
A375-P10
D6
A-375 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 695.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 192.03 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 280.62
A375-P10
D7
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1459.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 335.75 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1057.98
A375-P8
E6
A-375 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 717.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 192.77 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 88.65
A375-P8
D7
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 990.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.59 NGFR rabbit IgG(H+L) / donkey NGFR 219.19
Colo858-P7
F7
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1406.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 309.55 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 526.10
A375-P8
E7
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1030.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 218.08 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 94.73
A375-P8
F7
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1034.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 216.98 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 94.05
A375-P8
F6
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1063.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.81 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 77.29
Colo858-P8
B7
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1278.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 266.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 287.39
A375-P9
B7
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1301.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 298.21 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 167.81
A375-P10
E6
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1255.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 286.02 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 186.22
A375-P10
E7
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1296.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 279.58 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 298.21
A375-P9
E6
WM-115 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 699.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 101.58 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 189.85
WM115-P10
C7
WM-115 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 728.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 125.51 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 240.11
WM115-P10
C6