Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1586.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 273.96 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5368.53
Colo858-P2
E7
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 576.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.69 NGFR rabbit IgG(H+L) / donkey NGFR 331.16
A375-P1
G7
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 340.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.56 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 534.18
Colo858-P2
G6
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 262.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.10 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 594.35
Colo858-P2
D6
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 333.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.51 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 359.15
Colo858-P2
D7
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 576.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.13 NGFR rabbit IgG(H+L) / donkey NGFR 458.75
A375-P1
G6
A-375 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 356.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 402.10
A375-P3
F7
A-375 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 407.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 470.31
A375-P3
F6
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.28 NGFR rabbit IgG(H+L) / donkey NGFR 282.18
A375-P1
D7
WM-115 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 495.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 116.67 NGFR rabbit IgG(H+L) / donkey NGFR 2045.07
WM115-P1
G6
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 514.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.17 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1049.61
Colo858-P2
C6
COLO 858 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 394.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 181.23 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 556.52
Colo858-P2
C7
WM-115 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 535.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.93 NGFR rabbit IgG(H+L) / donkey NGFR 1519.23
WM115-P1
F6
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 308.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.67 NGFR rabbit IgG(H+L) / donkey NGFR 468.47
A375-P1
D6
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1330.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 270.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 275.47
A375-P3
E7
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 489.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.65 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 265.38
Colo858-P4
G6
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2087.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 364.28 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 315.96
A375-P3
E6
WM-115 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 488.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.79 NGFR rabbit IgG(H+L) / donkey NGFR 1294.99
WM115-P1
F7
COLO 858 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2275.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 321.46 NGFR rabbit IgG(H+L) / donkey NGFR 113.83
Colo858-P1
E6
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 717.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 197.50 NGFR rabbit IgG(H+L) / donkey NGFR 163.65
Colo858-P1
F6
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 216.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.80 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 468.99
A375-P3
D7
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.73 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 198.34
Colo858-P4
G7
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.82 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 499.78
A375-P3
D6
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 422.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 168.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 249.19
Colo858-P4
F6
WM-115 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 605.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 171.47 NGFR rabbit IgG(H+L) / donkey NGFR 818.62
WM115-P1
E6