Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.28 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1134.30
A375-P2
C10
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 381.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.57 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 194.41
A375-P4
D11
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 255.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 125.55 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 223.89
A375-P4
D10
COLO 858 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 604.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 186.18 NGFR rabbit IgG(H+L) / donkey NGFR 56.38
Colo858-P1
D10
WM-115 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 805.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 205.67 NGFR rabbit IgG(H+L) / donkey NGFR 546.41
WM115-P1
E10
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3595.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 449.49 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6455.59
Colo858-P2
E11
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 460.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 157.29 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 607.39
A375-P2
D10
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1734.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 353.69 NGFR rabbit IgG(H+L) / donkey NGFR 119.95
A375-P1
E11
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3740.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 484.10 NGFR rabbit IgG(H+L) / donkey NGFR 116.54
Colo858-P1
E10
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 641.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 176.08 NGFR rabbit IgG(H+L) / donkey NGFR 547.42
WM115-P1
E11
WM-115 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 693.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.81 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2535.44
WM115-P2
B10
WM-115 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 162.62 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 83.55
WM115-P4
B10
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 624.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 192.72 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 999.96
Colo858-P2
C10
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2453.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 457.23 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7382.49
A375-P2
B6
COLO 858 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1846.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 234.83 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 111.79
Colo858-P4
B7
COLO 858 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2379.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 269.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 102.93
Colo858-P4
B6
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1527.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 297.11 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 183.05
A375-P4
B7
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 543.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.84 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 248.68
Colo858-P4
C6
WM-115 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 502.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.72 NGFR rabbit IgG(H+L) / donkey NGFR 1551.41
WM115-P1
C6
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2059.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 371.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 198.06
A375-P4
B6
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 408.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 145.99 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 487.91
Colo858-P3
G7
WM-115 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 789.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 204.73 NGFR rabbit IgG(H+L) / donkey NGFR 834.68
WM115-P1
B6
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 637.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 199.79 NGFR rabbit IgG(H+L) / donkey NGFR 134.41
Colo858-P1
C6
COLO 858 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 284.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 204.79
Colo858-P4
C7
WM-115 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 684.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 192.63 NGFR rabbit IgG(H+L) / donkey NGFR 721.67
WM115-P1
B7