Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 52.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 137.72
WM115-P10
G10
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 67.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 50.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 134.52
WM115-P10
G11
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 74.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 151.25
WM115-P10
G2
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 442.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 146.15
WM115-P10
G3
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 425.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.95 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 161.81
WM115-P10
G4
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 525.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 97.59 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 157.54
WM115-P10
G5
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 592.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 91.98 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 197.93
WM115-P10
G6
WM-115 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 762.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 102.82 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 169.02
WM115-P10
G7
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 77.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 48.25
WM115-P10
G8
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 79.73 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 39.13
WM115-P10
G9
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3971.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 473.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 136.32
Colo858-P3
E10
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3639.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 456.21 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 112.58
Colo858-P3
E11
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2508.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 298.52 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 135.52
Colo858-P3
E2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2543.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 325.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 151.83
Colo858-P3
E3
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 436.76 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.84 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 955.43
Colo858-P3
E4
COLO 858 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 387.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 969.99
Colo858-P3
E5
COLO 858 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2380.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 306.62 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 186.68
Colo858-P3
E6
COLO 858 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1765.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 268.42 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 283.16
Colo858-P3
E7
COLO 858 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2416.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 295.01 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 178.42
Colo858-P3
E8
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1809.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 326.06 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 287.06
Colo858-P3
E9
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 509.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 192.18
Colo858-P3
F10
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 733.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 204.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 138.01
Colo858-P3
F11
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1053.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 226.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 692.16
Colo858-P3
F2
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 986.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 219.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 678.93
Colo858-P3
F3
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 401.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 984.71
Colo858-P3
F4