Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1330.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 270.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 275.47
A375-P3
E7
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 373.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 145.58 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 360.58
A375-P3
F10
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 438.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 318.75
A375-P3
F11
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 726.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.07 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 617.98
A375-P3
F3
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.41 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 713.04
A375-P3
F4
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2099.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 381.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 208.91
A375-P3
B10
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1738.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 333.51 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 159.52
A375-P3
B11
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2544.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 399.02 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 453.70
A375-P3
B3
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 316.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.63 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 839.25
A375-P3
B4
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1635.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 318.22 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 316.83
A375-P3
B7
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2118.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 373.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 311.40
A375-P3
B8
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 354.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.46 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 364.71
A375-P3
C10
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 449.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.05 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 327.81
A375-P3
C11
A-375 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 222.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 866.10
A375-P3
C5
A-375 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 409.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.93 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 534.93
A375-P3
C6
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 125.96 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 512.01
A375-P3
C8
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 278.03
A375-P3
C9
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 588.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.19 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 576.48
A375-P3
D2
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 497.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 556.24
A375-P3
D3
A-375 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 240.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 931.16
A375-P3
D5
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.82 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 499.78
A375-P3
D6
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1514.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 313.01 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 156.02
A375-P3
E11
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2735.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 434.01
A375-P3
E2
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.67 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 278.95
A375-P3
F9
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 393.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 134.83 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 503.03
A375-P3
F8