Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 97.15 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1106.45
WM115-P2
G8
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 343.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 432.43
Colo858-P2
G8
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 487.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.16 NGFR rabbit IgG(H+L) / donkey NGFR 1552.62
WM115-P7
G8
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 78.88 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 452.14
WM115-P9
G8
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 487.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.46 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 583.71
A375-P8
G8
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 77.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 48.25
WM115-P10
G8
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 407.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.43 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 634.29
Colo858-P3
G8
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 283.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.66 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 82.01
A375-P10
G8
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 447.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.74 NGFR rabbit IgG(H+L) / donkey NGFR 2263.76
WM115-P1
G8
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 363.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 79.92 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 368.38
WM115-P8
G8
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 372.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 261.05
Colo858-P4
G8
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 448.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.13 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 635.30
A375-P2
G8
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 457.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.63 NGFR rabbit IgG(H+L) / donkey NGFR 310.49
A375-P7
G8
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 410.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 360.95
Colo858-P9
G8
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.01 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 228.77
A375-P4
G8
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1152.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 197.53 NGFR rabbit IgG(H+L) / donkey NGFR 216.41
Colo858-P7
G8
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 424.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 142.27 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 309.39
A375-P2
G9
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 257.41
Colo858-P4
G9
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 294.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 351.08
WM115-P9
G9
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 91.80 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 187.51
WM115-P8
G9
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 370.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 125.66 NGFR rabbit IgG(H+L) / donkey NGFR 1337.75
WM115-P1
G9
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 407.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 431.83
Colo858-P3
G9
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.72 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 67.57
A375-P10
G9
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 342.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.69 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 261.12
Colo858-P2
G9
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 452.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.71 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 425.14
A375-P8
G9