Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 299.89
A375-P4
F8
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 321.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.06 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3169.91
WM115-P2
F9
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 321.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.80 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 224.82
A375-P4
F9
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 515.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.53 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 579.91
A375-P2
F9
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 538.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 185.81 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 204.99
Colo858-P9
F9
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 519.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.32 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 893.09
Colo858-P2
F9
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 390.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 171.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 67.02
Colo858-P10
F9
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 535.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.45 NGFR rabbit IgG(H+L) / donkey NGFR 1423.79
WM115-P7
F9
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 418.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 77.17
A375-P10
F9
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 343.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.55 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 244.04
WM115-P8
F9
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.33 NGFR rabbit IgG(H+L) / donkey NGFR 510.86
A375-P1
F9
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 684.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 231.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 929.36
A375-P8
F9
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 80.75 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 83.91
WM115-P4
F9
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 42.90
WM115-P10
F9
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 250.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 166.78
WM115-P3
F9
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.50 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 209.21
A375-P9
F9
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 563.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.96 NGFR rabbit IgG(H+L) / donkey NGFR 185.32
A375-P7
F9
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 616.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 190.31 NGFR rabbit IgG(H+L) / donkey NGFR 104.72
Colo858-P1
F9
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 465.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.37 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 505.86
Colo858-P3
F9
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 388.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.90 NGFR rabbit IgG(H+L) / donkey NGFR 1241.39
WM115-P1
F9
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.67 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 278.95
A375-P3
F9
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 82.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 396.60
WM115-P9
F9
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 422.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.92 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 244.29
Colo858-P4
F9
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1087.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.19 NGFR rabbit IgG(H+L) / donkey NGFR 120.28
Colo858-P7
F9
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 501.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.34 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 629.99
Colo858-P8
F9