Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3383.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 405.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 96.01
Colo858-P3
B11
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 902.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 205.80 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 258.98
Colo858-P9
B11
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1854.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.60 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7390.58
A375-P2
B11
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2000.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 389.89 NGFR rabbit IgG(H+L) / donkey NGFR 116.46
A375-P1
B11
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 199.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 101.49 NGFR rabbit IgG(H+L) / donkey NGFR 1348.46
WM115-P7
B11
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 99.35 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2839.07
WM115-P8
B11
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 56.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 66.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 93.13
WM115-P9
B11
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 479.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 157.95 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 156.44
WM115-P3
B11
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3387.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 414.22 NGFR rabbit IgG(H+L) / donkey NGFR 87.43
Colo858-P1
B11
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3107.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.20 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5963.42
Colo858-P2
B11
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 580.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.73 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 143.65
A375-P9
B11
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1425.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 200.45 NGFR rabbit IgG(H+L) / donkey NGFR 73.01
Colo858-P7
B11
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 409.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.05 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2109.14
WM115-P2
B11
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 82.98
WM115-P4
B11
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1686.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 314.79 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 140.38
A375-P4
B11
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 554.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 184.01 NGFR rabbit IgG(H+L) / donkey NGFR 580.80
WM115-P1
B11
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 673.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 179.29 NGFR rabbit IgG(H+L) / donkey NGFR 398.37
A375-P7
B11
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1738.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 333.51 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 159.52
A375-P3
B11
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 588.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 186.71 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6337.02
A375-P8
B11
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3490.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 412.36 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 99.67
Colo858-P4
B11
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 693.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.13 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 99.11
Colo858-P10
B11
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 109.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.20 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 60.22
WM115-P10
B11
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 573.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.42 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 113.14
A375-P10
B11
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2597.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 404.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 265.99
A375-P4
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3036.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 535.40 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6341.83
A375-P2
B2