Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 53.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 55.02 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 347.78
WM115-P9
C11
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 72.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 631.06
Colo858-P9
C11
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 873.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 240.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 169.62
Colo858-P3
C11
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.50 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 196.46
A375-P10
C11
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 227.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 99.02 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1461.45
A375-P8
C11
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -64.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.44 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 296.56
Colo858-P10
C11
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 438.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.41 NGFR rabbit IgG(H+L) / donkey NGFR 1154.86
WM115-P1
C11
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 68.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.16 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 454.32
Colo858-P8
C11
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 953.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 216.44 NGFR rabbit IgG(H+L) / donkey NGFR 57.05
Colo858-P1
C11
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 752.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.15 NGFR rabbit IgG(H+L) / donkey NGFR 792.07
Colo858-P7
C11
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.79 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 623.81
WM115-P2
C11
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 590.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 185.34 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1241.54
A375-P2
C11
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 70.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 55.30 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 164.98
WM115-P10
C11
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 684.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.91 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 357.40
A375-P4
C2
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 610.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.72 NGFR rabbit IgG(H+L) / donkey NGFR 258.83
A375-P7
C2
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 658.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.52 NGFR rabbit IgG(H+L) / donkey NGFR 784.50
A375-P1
C2
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 803.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 235.00 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 447.38
Colo858-P8
C2
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 593.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.33 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1088.62
A375-P8
C2
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 770.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 660.50
A375-P3
C2
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1193.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 206.40 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 350.18
Colo858-P4
C2
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.82 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 375.04
A375-P9
C2
WM-115 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 478.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.15 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 193.46
WM115-P10
C2
WM-115 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 651.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.57 NGFR rabbit IgG(H+L) / donkey NGFR 1715.91
WM115-P7
C2
WM-115 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 515.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 124.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 237.08
WM115-P4
C2
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1303.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 244.16 NGFR rabbit IgG(H+L) / donkey NGFR 525.63
Colo858-P1
C2