Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.73 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 657.31
Colo858-P9
C10
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 354.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.46 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 364.71
A375-P3
C10
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 739.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 194.15 NGFR rabbit IgG(H+L) / donkey NGFR 54.21
Colo858-P1
C10
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 479.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.80 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 562.92
WM115-P2
C10
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 641.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 182.89 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 187.13
Colo858-P3
C10
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 180.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 122.78 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 530.33
Colo858-P8
C10
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 555.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 110.85
Colo858-P4
C10
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.61 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 266.72
A375-P10
C10
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -32.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 96.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 324.72
Colo858-P10
C10
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.37 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 398.64
WM115-P9
C10
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 75.38 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1286.81
WM115-P8
C10
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 100.46 NGFR rabbit IgG(H+L) / donkey NGFR 1930.11
WM115-P7
C10
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 527.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 148.90 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 248.29
WM115-P3
C10
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.28 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1134.30
A375-P2
C10
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 175.13
A375-P4
C11
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 559.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.24 NGFR rabbit IgG(H+L) / donkey NGFR 158.24
A375-P1
C11
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 438.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.41 NGFR rabbit IgG(H+L) / donkey NGFR 1154.86
WM115-P1
C11
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 227.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 99.02 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1461.45
A375-P8
C11
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 178.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 285.72
A375-P9
C11
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 297.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.57 NGFR rabbit IgG(H+L) / donkey NGFR 358.16
A375-P7
C11
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 70.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 55.30 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 164.98
WM115-P10
C11
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -64.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.44 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 296.56
Colo858-P10
C11
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 72.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 631.06
Colo858-P9
C11
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 449.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.05 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 327.81
A375-P3
C11
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 247.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 102.49 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 112.45
WM115-P4
C11