Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 914.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 249.27 NGFR rabbit IgG(H+L) / donkey NGFR 1096.13
WM115-P7
E8
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 572.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.44 NGFR rabbit IgG(H+L) / donkey NGFR 1097.04
Colo858-P1
C5
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 97.15 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1106.45
WM115-P2
G8
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 613.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.35 NGFR rabbit IgG(H+L) / donkey NGFR 1111.88
Colo858-P1
E4
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1073.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 215.52 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1115.43
Colo858-P2
C2
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1228.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 363.06 NGFR rabbit IgG(H+L) / donkey NGFR 1118.25
WM115-P7
B2
WM-115 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 638.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1118.36
WM115-P8
B4
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 582.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.15 NGFR rabbit IgG(H+L) / donkey NGFR 1119.00
Colo858-P1
D4
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 557.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.39 NGFR rabbit IgG(H+L) / donkey NGFR 1122.76
WM115-P7
D5
WM-115 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 638.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.51 NGFR rabbit IgG(H+L) / donkey NGFR 1122.90
WM115-P7
E4
A-375 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 463.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.22 NGFR rabbit IgG(H+L) / donkey NGFR 1124.92
A375-P1
C5
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 644.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.52 NGFR rabbit IgG(H+L) / donkey NGFR 1131.58
WM115-P7
C5
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 876.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.24 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1131.61
Colo858-P2
C3
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 539.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.41 NGFR rabbit IgG(H+L) / donkey NGFR 1132.49
A375-P1
E4
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.28 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1134.30
A375-P2
C10
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 702.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 179.40 NGFR rabbit IgG(H+L) / donkey NGFR 1135.60
Colo858-P1
F4
A-375 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 370.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.10 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1138.09
A375-P8
D10
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 337.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 105.57 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1142.99
WM115-P2
D8
WM-115 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1060.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.41 NGFR rabbit IgG(H+L) / donkey NGFR 1143.08
WM115-P7
B6
A-375 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 306.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.82 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1150.78
A375-P8
G10
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.94 NGFR rabbit IgG(H+L) / donkey NGFR 1151.49
A375-P1
C4
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 438.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.41 NGFR rabbit IgG(H+L) / donkey NGFR 1154.86
WM115-P1
C11
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1091.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 202.38 NGFR rabbit IgG(H+L) / donkey NGFR 1156.82
WM115-P7
B7
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 663.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 168.59 NGFR rabbit IgG(H+L) / donkey NGFR 1157.13
Colo858-P1
C4
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 528.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 162.82 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1172.55
A375-P8
F8