Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 426.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.21 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 66.75
Colo858-P8
F5
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 390.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 171.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 67.02
Colo858-P10
F9
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 67.20
WM115-P10
B10
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.72 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 67.57
A375-P10
G9
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1343.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 187.72 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 68.93
Colo858-P10
B6
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 379.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.52 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 71.75
Colo858-P10
C8
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 400.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.11 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 72.10
Colo858-P10
F8
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 866.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.45 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 72.76
WM115-P10
B8
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1425.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 200.45 NGFR rabbit IgG(H+L) / donkey NGFR 73.01
Colo858-P7
B11
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 212.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.15 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 74.34
Colo858-P10
D8
COLO 858 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 685.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 210.02 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 74.53
Colo858-P8
C5
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 74.70
Colo858-P10
G9
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2172.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 364.23 NGFR rabbit IgG(H+L) / donkey NGFR 74.98
Colo858-P1
B9
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 908.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.71 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 75.37
A375-P8
C7
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 369.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 75.47
A375-P10
D9
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1117.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 75.66
Colo858-P10
E6
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 860.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 194.82 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 75.80
A375-P8
G6
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 418.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 77.17
A375-P10
F9
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1063.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.81 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 77.29
Colo858-P8
B7
COLO 858 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 395.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 162.41 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 77.85
Colo858-P8
G4
WM-115 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 927.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.15 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 78.35
WM115-P8
B6
COLO 858 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2886.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 315.93 NGFR rabbit IgG(H+L) / donkey NGFR 78.49
Colo858-P7
E5
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 830.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.02 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 78.71
Colo858-P8
E7
WM-115 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 232.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 79.09
WM115-P4
E9
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 487.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.79 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 79.46
Colo858-P4
D11