Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 77.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 48.25
WM115-P10
G8
COLO 858 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 681.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 208.24 NGFR rabbit IgG(H+L) / donkey NGFR 48.54
Colo858-P1
G10
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 206.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 78.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 51.00
WM115-P10
D8
COLO 858 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 293.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.53 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 52.78
Colo858-P8
D7
WM-115 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 752.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.35 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 52.89
WM115-P8
E6
COLO 858 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 308.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.51 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 54.15
Colo858-P8
C6
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 739.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 194.15 NGFR rabbit IgG(H+L) / donkey NGFR 54.21
Colo858-P1
C10
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 654.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 191.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 55.00
WM115-P10
B9
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 627.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 181.70 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 55.58
WM115-P10
E9
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 922.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 217.82 NGFR rabbit IgG(H+L) / donkey NGFR 56.24
Colo858-P1
F11
COLO 858 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 604.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 186.18 NGFR rabbit IgG(H+L) / donkey NGFR 56.38
Colo858-P1
D10
WM-115 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 342.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 84.72 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 56.65
WM115-P10
F8
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 636.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 217.87 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 56.99
Colo858-P8
D5
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 953.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 216.44 NGFR rabbit IgG(H+L) / donkey NGFR 57.05
Colo858-P1
C11
COLO 858 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 242.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.62 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 57.07
Colo858-P8
D6
COLO 858 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 302.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.83 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 57.65
Colo858-P8
F6
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 419.76 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.79 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 59.36
Colo858-P8
C7
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 109.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.20 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 60.22
WM115-P10
B11
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 718.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 187.45 NGFR rabbit IgG(H+L) / donkey NGFR 61.02
Colo858-P1
F10
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 443.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 84.66 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 61.97
WM115-P8
D4
WM-115 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 787.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 121.82 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 62.59
WM115-P8
F6
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.89 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 62.75
WM115-P10
E11
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 431.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 84.58 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 64.14
WM115-P8
G4
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 195.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 77.41 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 65.04
WM115-P10
E10
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 773.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 218.55 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 66.64
WM115-P10
E8