Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 94.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 151.92
WM115-P4
F5
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1121.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 267.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 152.07
A375-P10
E5
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1101.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.56 NGFR rabbit IgG(H+L) / donkey NGFR 152.71
Colo858-P7
C9
COLO 858 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2097.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 302.09 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 153.69
Colo858-P9
E4
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 471.76 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.07 NGFR rabbit IgG(H+L) / donkey NGFR 153.81
A375-P1
G11
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1339.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 253.18 NGFR rabbit IgG(H+L) / donkey NGFR 154.21
A375-P1
B9
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 235.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 87.45 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 154.60
WM115-P4
G8
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 600.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.75 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 154.70
A375-P9
E11
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 154.85
WM115-P4
E4
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 550.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.79 NGFR rabbit IgG(H+L) / donkey NGFR 155.36
A375-P1
F10
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 436.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.52 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 156.01
WM115-P10
D3
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1514.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 313.01 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 156.02
A375-P3
E11
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 479.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 157.95 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 156.44
WM115-P3
B11
WM-115 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 94.54 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 156.69
WM115-P4
B5
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 525.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 97.59 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 157.54
WM115-P10
G5
WM-115 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 737.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.67 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 157.61
WM115-P4
B3
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1683.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 315.88 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 157.68
Colo858-P4
E9
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 559.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.24 NGFR rabbit IgG(H+L) / donkey NGFR 158.24
A375-P1
C11
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 444.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 100.14 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 158.41
WM115-P10
F5
WM-115 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 927.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.29 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 158.90
WM115-P10
B6
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 102.23 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 158.98
WM115-P4
D5
WM-115 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 428.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.62 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 159.38
WM115-P4
E6
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1738.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 333.51 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 159.52
A375-P3
B11
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 61.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 159.57
WM115-P10
D10
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 824.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 179.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 159.69
WM115-P9
B2